diamide and Infertility--Male

To investigate the influence of human sperm nuclear chromatin on fertilization.. Prospective study.. Assisted reproductive technology unit at a university teaching hospital.. Fifty men starting an IVF-ET program.. Epifluorescent microscopic observation of human-ejaculated sperm nuclei stained with diamide-acridine orange. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) analysis of extracted sperm nucleoproteins.. Usefulness of diamide-acridine orange in analysis of human sperm nuclear chromatin and fertilization ability.. There was no correlation between the semen parameters and the diamide-acridine orange observation. A positive correlation was observed between the fertilization rate after conventional IVF and the green-type increase ratio (percentage of green-pattern sperm after diamide-acridine orange staining/percentage of green-pattern sperm after acridine orange staining). Furthermore, it was suggested by SDS-PAGE that structural differences were noticed between the fertile men and the men with sperm immaturity diagnosed after diamide-acridine orange staining.. Diamide-acridine orange staining was a more precise method for detecting chromatin abnormalities in human-ejaculated sperm and evaluating fertilization ability than acridine orange staining alone. This method can be used as a diagnostic tool to assess the fertilization ability of human-ejaculated spermatozoa before IVF procedures.

Topics: Acridine Orange; Chromatin; Diamide; Electrophoresis, Polyacrylamide Gel; Embryo Transfer; Female; Fluorescent Dyes; Humans; Infertility, Male; Male; Microscopy, Fluorescence; Nucleoproteins; Pregnancy; Prospective Studies; Protamines; Regression Analysis; Sperm Injections, Intracytoplasmic; Spermatozoa; Staining and Labeling