diamide and Diabetes-Mellitus--Type-2

A novel microflow technique is used to demonstrate that a weakened oxidant defense system found in diabetic erythrocytes leads to decreased levels of deformation-induced release of adenosine triphosphate (ATP) from erythrocytes. Addition of an oxidant to rabbit erythrocytes resulted in a 63% decrease in deformation-induced ATP release before eventually recovering to a value that was statistically equivalent to the initial value. Inhibition of glucose-6-phosphate dehydrogenase prevents recovery from the oxidant attack. Finally, results indicated that the ATP release from the erythrocytes of type II diabetics (91 nM +/- 10 nM) was less than half of that measured from the erythrocytes of healthy controls (190 +/- 10 nM). These data suggest that the antioxidant status of erythrocytes is a critical determinant in the ability of these cells to release ATP, a known nitric oxide stimulus.

Topics: Adenosine Triphosphate; Animals; Case-Control Studies; Cattle; Cell Size; Dehydroepiandrosterone; Diabetes Mellitus, Type 2; Diamide; Dose-Response Relationship, Drug; Endothelium, Vascular; Enzyme Inhibitors; Erythrocytes; Glucosephosphate Dehydrogenase; Glutathione; Humans; Male; Models, Biological; Nitric Oxide; Oxidants; Oxidation-Reduction; Pulmonary Artery; Rabbits; Stress, Mechanical; Sulfhydryl Reagents; Time Factors