deoxycholic-acid and Strongyloidiasis

The objective of this study was to identify soluble protein antigens that would induce protective immunity against infective-stage larvae (L-3) of Strongyloides stercoralis in mice. Deoxycholate (DOC)-soluble proteins derived from L-3, adsorbed to aluminum hydroxide, induced protective immunity in BALB/c mice. The immunized mice generated parasite-specific IgG that could transfer passive immunity to naïve animals. The protective antibodies bound to parasite antigens found in the muscles and nerve cords of the L-3. An IgG affinity chromatography column generated with IgG from the sera of DOC-immunized mice was used to purify specific larval antigens. Proteins were eluted from the affinity column with sizes of 80, 75, 61, 57, 43, and 32 kDa. This antigen pool stimulated both proliferation and IL-5 production by splenocytes recovered from mice immunized with live L-3. Vaccination of mice with the immunoaffinity-isolated antigens led to significant protective immunity, with 83% of challenge larvae killed. This study demonstrates that IgG-isolated proteins are candidate antigens for a vaccine against larval S. stercoralis.

Topics: Animals; Antigens, Helminth; Chromatography, Affinity; Deoxycholic Acid; Helminth Proteins; Immune Sera; Immunization, Passive; Immunoglobulin G; Interleukin-5; Larva; Male; Mice; Mice, Inbred BALB C; Microscopy, Immunoelectron; Solubility; Strongyloides stercoralis; Strongyloidiasis; T-Lymphocytes