deoxycholic-acid and Periodontal-Diseases

The study evaluated the effect of 2% sodium desoxycholate combined with whole plasma from a single donor on gingival fibroblast attachment to diseased root surfaces. Twenty extracted periodontally involved teeth were cut into halves buccolingually and sterilized by moist heat under high pressure. The diseased root surface of each control half was rubbed with a sterile cotton pellet saturated with Dulbecco's phosphate-buffered saline (PBS). The diseased root surface of each experimental half was rubbed with a sterile cotton pellet saturated with 2% sodium desoxycholate and then rubbed with a pellet soaked in human plasma. The control and experimental halves were placed in separate petri dishes, and a fibroblast cell suspension was added to each dish. The mean number of attached cells per half tooth was calculated for each group. Tooth surfaces treated with PBS (controls) showed a mean of 307 +/- 63 attached cells for 17 tooth halves; the experimental treated surfaces exhibited a mean of 650 +/- 130 attached cells for 16 halves. The difference between these numbers was statistically significant (P less than 0.01). These findings suggest that the desoxycholate/plasma combination enhanced in vitro fibroblast attachment to diseased root surfaces.

Topics: Adult; Cell Adhesion; Cell Count; Cells, Cultured; Deoxycholic Acid; Fibroblasts; Gingiva; Humans; Periodontal Diseases; Tooth Root

Topics: Blood Proteins; Citrates; Citric Acid; Dental Scaling; Deoxycholic Acid; Edetic Acid; Endotoxins; Humans; Immunosuppressive Agents; Periodontal Diseases; Sodium Hypochlorite; Tooth Root

This study evaluated the effects of chemical and biologic agents on periodontally diseased root surfaces which had been scaled or root planed. The proximal surfaces of 25 teeth were scaled to remove all visible calculus, and the proximal surfaces of another 25 teeth were vigorously root planed to remove all cementum and to achieve a hard, smooth, glass-like surface. Five scaled and five root planed specimens were randomly selected for light microscopic examination to determine the amount of cementum removed. Cementum remained on all scaled surfaces but root planing had removed most of the more coronal cementum. Each of the 40 remaining teeth (20 scaled and 20 root planed) was longitudinally sectioned to obtain an experimental and control specimen. Four scaled and four root planed sections were randomly selected as experimental specimens for a test of each of the following five agents: (1) saturated citric acid for three minutes; (2) 15% EDTA for 5 minutes; (3) sodium hypochlorite for 5 minutes, followed by a 30-second application of 5% citric acid; (4) sodium hypochlorite alone for 5 minutes; and (5) 2% sodium deoxycholate (NAD) for 1 minute, followed by a 1-minute rinse in distilled water, and then a 1-minute application of 5% Cohn's fraction IV1. The control for each experimental specimen was treated with saline. All samples were prepared for SEM and examined at 3,000 X. Areas of particular interest were also examined at 12,000 X. The chemical treatments exposed only individual collagen fibers or irregular fiber bundles on the scaled surfaces. Saturated citric acid, EDTA, and sodium hypochlorite with citric acid neutralization removed debris and exposed openings in the root surfaces. Sodium hypochlorite alone and NaD/Cohn's fraction IV1 were less effective in removing surface debris and had an effect similar to that seen in the saline controls. Application to root planed specimens of saturated citric acid, EDTA, and sodium hypochlorite followed by 30 seconds of citric acid neutralization resulted in surfaces virtually free of debris and with numerous collagen fibers exposed on the surface. EDTA appeared to cause a morphologic change in the collagen fibers. Sodium hypochlorite alone, sodium deoxycholate followed by Cohn's fraction IV1, and physiologic saline were relatively ineffective in surface debridement.

Topics: Adult; Blood Proteins; Citrates; Citric Acid; Dental Scaling; Deoxycholic Acid; Edetic Acid; Endotoxins; Humans; Microscopy, Electron, Scanning; Periodontal Diseases; Pharmacology; Sodium Hypochlorite; Tooth Root

Untreated periodontally-involved teeth contain cementum-bound endotoxin, that may prevent periodontal new attachment during healing after pocket therapy. The purpose of this study was to restore biocompatibility to diseased root surfaces by a non-invasive treatment. Untreated human teeth removed for severe chronic periodontal disease were split buccolingually, the level of connective tissue was scribed on the roots, the specimens cleaned of plaque and visible calculus, and autoclaved. Cementum removal was not attempted. One member of each pair was treated with phosphate-buffered saline as a control. Sodium desoxycholate (2%), Cohn plasma fraction IV1 (5%), or deoxycholate followed by plasma fraction were applied for one minute. Roots were incubated in a suspension of gingival fibroblasts for 48 hours, then rinsed, fixed, and stained. Counts were made of the number of attached cells at 40 x magnification using an ocular grid. There was a significant increase (p less than 0.01) of the combination treatment over controls. All treatments provided a biological surface for attachment of fibroblasts, in vitro, compared to untreated roots.

Topics: Alpha-Globulins; Cell Adhesion; Deoxycholic Acid; Endotoxins; Fibroblasts; Humans; Periodontal Diseases; Periodontium; Tooth Root