deoxycholic-acid and Cholera

The cells of P. multocida strain P-1059 were destroyed with the French pressure cell; the ribosomal fraction proven to be homogeneous by analytical ultracentrifugation was obtained from the product by centrifugal fractionation, zonal electrophoresis, and Sephadex G-200 gel filtration. The ribosomal fraction exhibited intense protective antigenicity in mice and chickens, but the lipopolysaccharide (endotoxin) and the other bacterial cell fraction obtained in this experiment did not. Sodium deoxycholate treatment of the ribosomal fraction resulted in only a 13% loss in immunological activity, and ribonuclease treatment caused a 60% loss of activity.

Topics: Animals; Antigens; Chickens; Cholera; Deoxycholic Acid; Male; Mice; Pasteurella; Pasteurella Infections; Ribosomal Proteins; Ribosomes; RNA, Ribosomal; Subcellular Fractions

Segments of rabbit ileum were examined by scanning electron microscopy (SEM) after exposure to various compounds known to stimulate fluid secretion in the small intestine. After perfusion with ricinoleate (castor oil) at a 10 mM concentration, striking changes were observed at villus tips and on the apicolateral surfaces of villi; erosions at tips were confirmed by light microscopy of the same pieces of tissue examined by SEM. The appearances after ricinoleate were reversed in part during perfusion with control buffer for 2 hr. Statistical comparisons between control perfused, treated, and reversal tissues showed that the "damage" produced by ricinoleate, and its reversal, was significant statistically. Tissues treated with other intestinal secretagogues, oleic acid (10 mM), deoxycholic acid (4 mM), cholic acid (4 mM), dioctyl sodium sulfosuccinate (6 mM), and cholera toxin (1 microgram/ml) also showed surface changes by SEM. Fluid secretion, produced by an osmotic agent (mannitol) was not associated with "damage." Changes in structure of the mucosal surface after exposure to intestinal secretagogues should be considered as one possible mechanism of impaired fluid transport.

Topics: Animals; Bile Acids and Salts; Biological Transport; Cholera; Cholic Acids; Deoxycholic Acid; Enterotoxins; Fatty Acids; Fatty Acids, Unsaturated; Ileum; Intestinal Mucosa; Male; Microscopy, Electron, Scanning; Octanols; Oleic Acids; Rabbits; Ricinoleic Acids; Stimulation, Chemical; Succinates

Topics: Amphotericin B; Animals; Biological Transport; Cholera; Cholesterol; Deoxycholic Acid; Dogs; Enterotoxins; Jejunum; Sodium; Stimulation, Chemical; Time Factors; Water

Topics: Agar; Cell Differentiation; Cholera; Citrates; Citric Acid; Culture Media; Deoxycholic Acid; Humans; Vibrio; Vibrio cholerae