deoxycholic-acid and Biliary-Fistula

Intestinal absorption and metabolism of 3,7-dioxo-5 beta-cholanoic acid, were studied in the bile fistula rats, hamsters, guinea-pigs and rabbits. The influence of dose (1 and 100 mg/kg) on the absorption and the metabolism was also estimated. The dioxo bile acid was absorbed efficiently from the intestine and quickly excreted into bile in these animals. Large dose did not retard the absorption rate and showed a significant choleretic effect for a few hours. Species differences were observed in the metabolism of this compound. In hamsters and guinea-pigs, most of the metabolites in the bile were conjugated with either taurine or glycine. The proportion of bile acids amidated with glycine was greater with the large dose. In rats, the biliary metabolites were conjugated with taurine, but not with glycine, whereas in rabbits, glycine conjugates were the only recovered metabolites. Unconjugated metabolites were also detected in the bile of the rodents, and the proportion of them rose to 17-29% after the administration of 100 mg/kg quantities. A small part of unchanged 3,7-dioxo-5 beta-cholanoic acid was excreted into the bile as both the conjugated and unconjugated forms in these animals. The greater part of this compound administered was metabolized to 7-ketolithocholic acid, chenodeoxycholic acid and ursodeoxycholic acid. In hamsters and guinea-pigs, chenodeoxycholic acid was a greater metabolite of this compound than ursodeoxycholic acid, while in rats and rabbits, the amount of ursodeoxycholic acid exceeded that of chenodeoxycholic acid. In rats, the resulting dihydroxy bile acids were further metabolized to alpha- and beta-muricholic acids.

Topics: Amidohydrolases; Animals; Biliary Fistula; Biotransformation; Chromatography, Thin Layer; Cricetinae; Deoxycholic Acid; Guinea Pigs; Hydrolysis; Intestinal Absorption; Male; Mesocricetus; Rabbits; Rats; Rats, Inbred Strains

The effect of changes of both the rate of secretion and the composition of bile acids on biliary proteins was studied in a bile fistula hamster model. Biliary protein secretion as well as bile flow and bile acid secretion were studied in response to intravenous infusions of low, medium and high doses of ursodeoxycholic acid and chenodeoxycholic acid in comparison to the infusion of the normal saline carrier (control) solution. The control-infused animals showed a marked and statistically significant increase in both the concentration and total excretion of biliary proteins. All three doses of ursodeoxycholic acid either prevented the increase of protein concentration or led to its decrease. The low and medium doses of chenodeoxycholic acid had similar effects. However, the high dose of this bile acid was cholestatic and increased the biliary protein concentration. The results of the study indicate that decreases in bile acid secretion, as they occur after an interruption of the enterohepatic circulation, may lead to major increases in biliary protein concentration. The study also shows that these changes in protein secretion, which may promote nucleation, are reversed by the cholelitholytic bile acids, ursodeoxycholic acid and chenodeoxycholic acid.

Topics: Animals; Bile; Bile Acids and Salts; Biliary Fistula; Chenodeoxycholic Acid; Cholic Acid; Cholic Acids; Chromatography, Gas; Cricetinae; Deoxycholic Acid; Disease Models, Animal; Lithocholic Acid; Male; Mesocricetus; Proteins; Ursodeoxycholic Acid

The effects of both apolipoprotein B,E receptor-dependent and receptor-independent uptake of low-density lipoprotein (LDL) in the liver on bile secretion were studied in bile fistula hamsters. Three groups of animals were studied after 4 wk of feeding either a control, chenodeoxycholic acid-, or ursodeoxycholic acid-containing diet. The hepatic receptor-dependent and receptor-independent uptake of LDL was related to both bile flow and biliary lipid secretion. The correlation with bile flow and biliary lipid secretion was positive for the receptor-dependent, but negative for the receptor-independent uptake of LDL. Although the receptor-mediated LDL uptake appeared to exert a strong influence on bile acid-independent bile flow, the receptor-independent uptake showed a significant relation with biliary bile acid excretion. Differences between the two mechanisms of LDL uptake were also evident in the biliary bile acid-cholesterol coupling, which was significantly stronger during receptor-independent than during receptor-dependent uptake of LDL. The effects of LDL uptake on bile secretion were modulated by the experimentally induced changes in both the content and composition of bile acids in the enterohepatic circulation.

Topics: Animals; Apolipoproteins; Bile; Biliary Fistula; Chenodeoxycholic Acid; Cricetinae; Deoxycholic Acid; Diet; Enterohepatic Circulation; Lipoproteins, LDL; Liver; Male; Mesocricetus; Receptors, Cell Surface; Receptors, Lipoprotein; Ursodeoxycholic Acid

Topics: Acute Disease; Adolescent; Adult; Bile; Bile Acids and Salts; Biliary Fistula; Chenodeoxycholic Acid; Cholic Acids; Deoxycholic Acid; Glycocholic Acid; Hepatitis A; Humans; Liver; Liver Cirrhosis; Liver Diseases; Middle Aged; Taurocholic Acid

Topics: Animals; Biliary Fistula; Deoxycholic Acid; Injections, Intravenous; Intestinal Mucosa; Liver; Mice; Rats

Topics: Animals; Bile; Bile Acids and Salts; Bile Duct Diseases; Bile Ducts; Biliary Fistula; Cholagogues and Choleretics; Dehydrocholic Acid; Deoxycholic Acid; Dogs