demecolcine and Mouth-Neoplasms

demecolcine has been researched along with Mouth-Neoplasms* in 2 studies

Other Studies

2 other study(ies) available for demecolcine and Mouth-Neoplasms

Article	Year
Chromosomal instability and cytoskeletal defects in oral cancer cells. Proceedings of the National Academy of Sciences of the United States of America, 2000, Jan-04, Volume: 97, Issue:1 Oral squamous cell carcinomas are characterized by complex, often near-triploid karyotypes with structural and numerical variations superimposed on the initial clonal chromosomal alterations. We used immunohistochemistry combined with classical cytogenetic analysis and spectral karyotyping to investigate the chromosomal segregation defects in cultured oral squamous cell carcinoma cells. During division, these cells frequently exhibit lagging chromosomes at both metaphase and anaphase, suggesting defects in the mitotic apparatus or kinetochore. Dicentric anaphase chromatin bridges and structurally altered chromosomes with consistent long arms and variable short arms, as well as the presence of gene amplification, suggested the occurrence of breakage-fusion-bridge cycles. Some anaphase bridges were observed to persist into telophase, resulting in chromosomal exclusion from the reforming nucleus and micronucleus formation. Multipolar spindles were found to various degrees in the oral squamous cell carcinoma lines. In the multipolar spindles, the poles demonstrated different levels of chromosomal capture and alignment, indicating functional differences between the poles. Some spindle poles showed premature splitting of centrosomal material, a precursor to full separation of the microtubule organizing centers. These results indicate that some of the chromosomal instability observed within these cancer cells might be the result of cytoskeletal defects and breakage-fusion-bridge cycles. Topics: Antigens, Nuclear; Carcinoma, Squamous Cell; Cell Cycle Proteins; Centromere; Chromosome Aberrations; Chromosome Breakage; Chromosome Painting; Cytoskeleton; Demecolcine; Fluorescent Antibody Technique; Humans; Immunohistochemistry; Karyotyping; Mitosis; Mouth Neoplasms; Nuclear Matrix-Associated Proteins; Nuclear Proteins; Tubulin; Tumor Cells, Cultured	2000
Tumor inhibitors. 88. The antileukemia principles of Colchicum speciosum. Lloydia, 1973, Volume: 36, Issue:3 Topics: Animals; Antineoplastic Agents; Biological Assay; Carcinoma; Cell Line; Colchicine; Colchicum; Demecolcine; Drug Evaluation, Preclinical; Humans; Leukemia L1210; Mice; Mouth Neoplasms; Plants, Medicinal	1973

Article

Year

Chromosomal instability and cytoskeletal defects in oral cancer cells.

Proceedings of the National Academy of Sciences of the United States of America, 2000, Jan-04, Volume: 97, Issue:1

Oral squamous cell carcinomas are characterized by complex, often near-triploid karyotypes with structural and numerical variations superimposed on the initial clonal chromosomal alterations. We used immunohistochemistry combined with classical cytogenetic analysis and spectral karyotyping to investigate the chromosomal segregation defects in cultured oral squamous cell carcinoma cells. During division, these cells frequently exhibit lagging chromosomes at both metaphase and anaphase, suggesting defects in the mitotic apparatus or kinetochore. Dicentric anaphase chromatin bridges and structurally altered chromosomes with consistent long arms and variable short arms, as well as the presence of gene amplification, suggested the occurrence of breakage-fusion-bridge cycles. Some anaphase bridges were observed to persist into telophase, resulting in chromosomal exclusion from the reforming nucleus and micronucleus formation. Multipolar spindles were found to various degrees in the oral squamous cell carcinoma lines. In the multipolar spindles, the poles demonstrated different levels of chromosomal capture and alignment, indicating functional differences between the poles. Some spindle poles showed premature splitting of centrosomal material, a precursor to full separation of the microtubule organizing centers. These results indicate that some of the chromosomal instability observed within these cancer cells might be the result of cytoskeletal defects and breakage-fusion-bridge cycles.

Topics: Antigens, Nuclear; Carcinoma, Squamous Cell; Cell Cycle Proteins; Centromere; Chromosome Aberrations; Chromosome Breakage; Chromosome Painting; Cytoskeleton; Demecolcine; Fluorescent Antibody Technique; Humans; Immunohistochemistry; Karyotyping; Mitosis; Mouth Neoplasms; Nuclear Matrix-Associated Proteins; Nuclear Proteins; Tubulin; Tumor Cells, Cultured

2000

Tumor inhibitors. 88. The antileukemia principles of Colchicum speciosum.

Lloydia, 1973, Volume: 36, Issue:3

Topics: Animals; Antineoplastic Agents; Biological Assay; Carcinoma; Cell Line; Colchicine; Colchicum; Demecolcine; Drug Evaluation, Preclinical; Humans; Leukemia L1210; Mice; Mouth Neoplasms; Plants, Medicinal

1973