demecolcine and Leukemia--Myeloid

Human myeloblastic leukemia cell line ML-1 was induced to differentiate by 1 mumol/l all-trans-retinoic acid (RA) or by 1,25-dihydroxyvitamin D3 (1,25(OH)2D3). After 4-6 days of the induction several differentiation-associated characteristics were observed: (1) Ability to stimulate respiration burst in the ML-1 induced cells as detected by nitroblue tetrazolium (NBT) test or by chemiluminescence (CL). (The percentage of the NBT-positive cells was up to 99% in the RA-induced cells and up to 85% in the 1,25(OH)2D3-induced cells.) (2) Substantially higher phagocytosis of colloid iron, latex or Staphylococcus particles was found in the induced cells. (3) The 1,25(OH)2D3-induced ML-1 cells expressed the monocytic enzyme NaF-inhibitable alpha-naphthyl butyrate esterase and the surface monocytic antigen CD-14. (4) A majority of the induced cells lost the morphological features of blast cells; while the 1,25(OH)2D3-treated cells acquired certain features of monocyte-macrophage differentiation, the RA-treated cells displayed several granulocytic characteristics. (5) Cytofluorometric DNA assay after treatment of the cells with colcemid showed that the decline observed in the growth rate of the induced cells was connected with their arrest in G1/G0 phase of the cell cycle. The obtained results indicate granulocytic differentiation of the RA-induced ML-1 cells and monocyto/macrophage differentiation of the 1,25-(OH)2-D3 induced cells.

Topics: Acid Phosphatase; Alkaline Phosphatase; Antigens, Differentiation, Myelomonocytic; Calcitriol; Carboxylic Ester Hydrolases; Cell Differentiation; Cell Division; Demecolcine; Humans; Iron; Leukemia, Myeloid; Lipopolysaccharide Receptors; Naphthols; Oxygen Consumption; Peroxidase; Tretinoin

Topics: Bone Marrow Cells; Cells, Cultured; Chromosomes, Human, 21-22 and Y; Colony-Forming Units Assay; Demecolcine; Erythrocytes; Erythropoietin; Hematopoietic Stem Cells; Humans; Leukemia, Myeloid; Leukemia, Myeloid, Acute; Monocytes

Topics: Administration, Oral; Demecolcine; Drug Administration Schedule; Drug Evaluation; Female; Humans; Leukemia, Myeloid; Male; Remission, Spontaneous

Topics: Autoanalysis; Bleomycin; Cell Division; Cell Line; Cell Survival; Cytarabine; Dactinomycin; Demecolcine; DNA, Neoplasm; Histocytochemistry; Humans; Hydroxyurea; Leukemia, Myeloid; Methods; Microscopy, Fluorescence; Mitosis; Staining and Labeling; Thymidine; Time Factors; Tritium

Forty-three patients with chronic myeloid leukaemia have been treated with hydroxyurea in order to be subjected to leucopheresis for white cell transfusions. Hydroxyurea decreases leucocytosis when it is administered and the blood granulocyte number increases soon after the drug is stopped. The survival of the patients is not different from the survival of the patients treated with conventional chemotherapy (busulphan, mitobronitol) and it is superior to the survival of patients treated with external radiotherapy or with (32)P. Half of the patients were subjected to splenectomy during first remission for a phase II trial. They were not randomized, but the distribution according to age was similar in the two groups. A slight difference appears in favour of splenectomy so far as survival is concerned, but there were three post-operative deaths out of 18 patients. We conclude that a phase II trial on the value of splenectomy is indicated ethically, but that the patients should be operated on and nursed in a microbiologically controlled environment.

Topics: Adolescent; Adult; Aged; Blood Transfusion; Busulfan; Demecolcine; Female; Humans; Hydroxyurea; Leukemia, Myeloid; Leukocyte Count; Leukocytes; Leukocytosis; Male; Methods; Middle Aged; Splenectomy

Topics: Chronic Disease; Demecolcine; Humans; Leukemia; Leukemia, Myeloid; Polycythemia

Topics: Colchicine; Demecolcine; Disease; Leukemia; Leukemia, Myelogenous, Chronic, BCR-ABL Positive; Leukemia, Myeloid; Medical Records; Pregnancy; Ribs

Topics: Colchicine; Demecolcine; Leukemia; Leukemia, Myeloid

Topics: Busulfan; Colchicine; Demecolcine; Leukemia; Leukemia, Myelogenous, Chronic, BCR-ABL Positive; Leukemia, Myeloid

Topics: Colchicine; Demecolcine; Leukemia; Leukemia, Myelogenous, Chronic, BCR-ABL Positive; Leukemia, Myeloid

Topics: Colchicine; Demecolcine; Leukemia; Leukemia, Myeloid; Pregnancy

Topics: Colchicine; Demecolcine; Leukemia; Leukemia, Myelogenous, Chronic, BCR-ABL Positive; Leukemia, Myeloid

Topics: Antineoplastic Agents; Busulfan; Colchicine; Demecolcine; Glycols; Leukemia; Leukemia, Myeloid

Topics: Colchicine; Demecolcine; Leukemia; Leukemia, Myeloid; Neoplasms

Topics: Antineoplastic Agents; Colchicine; Demecolcine; Leukemia; Leukemia, Myeloid