decussatin and Leukemia--Promyelocytic--Acute

decussatin has been researched along with Leukemia--Promyelocytic--Acute* in 1 studies

Other Studies

1 other study(ies) available for decussatin and Leukemia--Promyelocytic--Acute

Article	Year
Anti-proliferation, cell cycle arrest and apoptosis induced by a natural xanthone from Gentianopsis paludosa Ma, in human promyelocytic leukemia cell line HL-60 cells. Toxicology in vitro : an international journal published in association with BIBRA, 2009, Volume: 23, Issue:3 1-hydroxy-3,7,8-trimethoxyxanthone (xanthone 1) was isolated from Gentianopsis paludosa Ma and identified by MS and NMR in our laboratory. In this study, the results showed that xanthone 1 is a potent inducer of anti-proliferation and apoptosis in HL-60 cells. When the cells treated with lower concentrations of xanthone 1 (12.4-74.4microM), significant proliferation inhibition was detected by cell viability assay and morphological analyses, and conspicuous G1 and G2/M cell cycle arrest were observed by flow cytometric (FCM) analysis. However, when the cells treated with higher doses of xanthone 1 (82.7-330.8microM), significant apoptosis was observed by double sequential AO/EB staining, DNA fragmentation assay and FCM analysis. In addition, conspicuous DNA damage was detected by comet assay. In short, all the results showed that xanthone 1 had a significant cytotoxic effect and could induce proliferation inhibition and apoptosis in HL-60 cells in a time- and dose-dependent manner. It was possible that xanthone 1 could induce DNA damage in HL-60 cells, which resulted in G1 phase arrest at the lower concentrations and G2/M phase arrest at the higher concentrations, thus inhibiting the cell proliferation, and irreparable DNA damage at the higher concentrations might be responsible for the occurrence of apoptosis. Topics: Antineoplastic Agents; Apoptosis; Cell Cycle; Cell Proliferation; Cell Survival; Comet Assay; DNA Damage; Drug Screening Assays, Antitumor; Gentianaceae; HL-60 Cells; Humans; Leukemia, Promyelocytic, Acute; Plant Extracts; Plants, Medicinal; Tibet; Xanthones	2009

Article

Year

Anti-proliferation, cell cycle arrest and apoptosis induced by a natural xanthone from Gentianopsis paludosa Ma, in human promyelocytic leukemia cell line HL-60 cells.

Toxicology in vitro : an international journal published in association with BIBRA, 2009, Volume: 23, Issue:3

1-hydroxy-3,7,8-trimethoxyxanthone (xanthone 1) was isolated from Gentianopsis paludosa Ma and identified by MS and NMR in our laboratory. In this study, the results showed that xanthone 1 is a potent inducer of anti-proliferation and apoptosis in HL-60 cells. When the cells treated with lower concentrations of xanthone 1 (12.4-74.4microM), significant proliferation inhibition was detected by cell viability assay and morphological analyses, and conspicuous G1 and G2/M cell cycle arrest were observed by flow cytometric (FCM) analysis. However, when the cells treated with higher doses of xanthone 1 (82.7-330.8microM), significant apoptosis was observed by double sequential AO/EB staining, DNA fragmentation assay and FCM analysis. In addition, conspicuous DNA damage was detected by comet assay. In short, all the results showed that xanthone 1 had a significant cytotoxic effect and could induce proliferation inhibition and apoptosis in HL-60 cells in a time- and dose-dependent manner. It was possible that xanthone 1 could induce DNA damage in HL-60 cells, which resulted in G1 phase arrest at the lower concentrations and G2/M phase arrest at the higher concentrations, thus inhibiting the cell proliferation, and irreparable DNA damage at the higher concentrations might be responsible for the occurrence of apoptosis.

Topics: Antineoplastic Agents; Apoptosis; Cell Cycle; Cell Proliferation; Cell Survival; Comet Assay; DNA Damage; Drug Screening Assays, Antitumor; Gentianaceae; HL-60 Cells; Humans; Leukemia, Promyelocytic, Acute; Plant Extracts; Plants, Medicinal; Tibet; Xanthones

2009