deamino-arginine-vasopressin and Peripheral-Vascular-Diseases

A radioimmunoassay (RIA) has been set up using purified human melanoma tissue plasminogen activator and a specific antiserum raised against it. Concentration of antigen in citrated plasma of resting human subjects was found to be 7.1 +/- 1.6 ng/ml (mean +/- S.D.) while the lower limit of sensitivity of the RIA was 2 ng/ml. After venous occlusion or DDAVP infusion the levels of antigen were invariably elevated and a significant increase in the proportion of antigen having fibrin binding properties was observed. Decay studies in vitro and gel filtration of post-stimulus plasma indicated that the RIA detects active and inactive forms of antigen and the possible nature of the inactive antigen is discussed.

Topics: Arm; Chromatography, Gel; Constriction, Pathologic; Deamino Arginine Vasopressin; Fibrin; Fibrinolysis; Hemostatics; Humans; Infusions, Parenteral; Male; Peripheral Vascular Diseases; Radioimmunoassay; Reproducibility of Results; Tissue Plasminogen Activator