cytochrome-c-t has been researched along with Urinary-Incontinence--Stress* in 1 studies
1 other study(ies) available for cytochrome-c-t and Urinary-Incontinence--Stress
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[Effects of 7-difluoromethy-5, 4'-dimethoxygenistein on
stress urinary incontinence model in rats and its mechanisms].
To investigate the effects of 7-difluoromethy-5, 4'-dimethoxygenistein (DFMG) on stress urinary incontinence (SUI) model in Sprague Dawley (SD) rats and its possible mechanisms. Methods: SD rat model of SUI was established through simulating pregnancy, birth trauma and ovarian castration. The rats were divided into a normal control group, a SUI group, and a DFMG group at 10 or 20 mg/kg. They were treated with 10 mg/kg normal saline (NS), 10 mg/kg NS, 10 mg/kg DFMG and 20 mg/kg DFMG, respectively, via gastric gavage every other day. Maximal bladder capacity (MBC), leak point pressure (LPP), abdominal leak point pressure (ALPP), hematoxylin-eosin (HE) staining, and Masson staining were performed to detect the index for the model. MiR-26b and its down-stream gene phosphatase and tensin homolog deleted on chromosome 10 (PENT) mRNA in urethral sphincter muscles cells (USMCs) were analyzed by RT-PCR. The protein levels of PENT, phosphatidylinositol 3-kinase (PI3K), protein kinaseB (AKT), B-cell lymphoma 2 (Bcl-2), Bcl-2 associated X protein (Bax), cytochrome C(Cyt-c) and caspase-3 were examined by Western blot. The apoptotic rate of USMCs was determined by flow cytometry (FCM), and the proliferative rate of USMCs was examined by MTT assay. Results: The SD rat model of SUI was successfully established. HE staining and Masson staining showed that the pathological features of urethral sphincter were improved in the DFMG-treated groups compared with the SUI group. The urine dynamics indexes of model rats, such as MBC, LPP and ALPP, were improved (all P﹤0.05). The results of RT-PCR showed that the miR-26b mRNA was up-regulated (P﹤0.05) and PENT mRNA was down-regulated (P﹤0.05) in the DFMG-treated groups compared with the SUI group. Simultaneously, compared with the SUI group, the protein levels of PENT, Bax, Cyt-c and caspase-3 were down-regulated (all P﹤0.05) and the protein levels of PI3K, AKT and Bcl-2 protein were up-regulated (all P﹤0.05), accompanied by the decreased apoptotic rate of USMCs (P﹤0.05) and the increased proliferative rate of USMCs (P﹤0.05) in the DFMG-treated groups. Conclusion: The DFMG can significantly improve the symptoms of urinary dynamics, which might be related to the up-regulation of miR-26b expression and the regulation of PI3/AKT-Bcl-2/ Bax signaling pathways.. 目的:探讨7-二氟亚甲基-5, 4'-二甲氧基金雀异黄素(7-difluoromethy-5, 4'-dimethoxygenistein,DFMG)对SD大鼠压力性尿失禁(stress urinary incontinence,SUI)模型的疗效及其机制。方法:采用模拟妊娠、难产产伤及卵巢去势建立SD大鼠SUI模型,分为正常对照组、SUI组、DFMG(10,20 mg/kg)组,模型鼠行DFMG隔日灌胃治疗。采用膀胱最大容积(maximal bladder capacity,MBC)、漏尿点压力(leak point pressure,LPP)、腹部漏尿点压力(abdominal leak point pressure,ALPP)以及HE染色和Masson染色检测建模效果;采用RT-PCR检测尿道括约肌细胞(urethral sphincter muscles cells,USMCs)miR-26b及其下游靶基因磷酸酶和肌腱同源染色体(phosphatase and tensin homolog deleted on chromosome 10,PENT)mRNA表达;用Western印迹检测USMCs细胞PENT,磷脂酰肌醇3激酶(phosphatidylinositol 3-kinase,PI3K),蛋白激酶B(protein kinase B,AKT),B细胞淋巴瘤/白血病-2(B-cell lymphoma 2,Bcl-2),Bcl-2相关X蛋白(Bcl-2 associated X protein,Bax),细胞色素C(cytochrome C,Cyt-C)和含半胱氨酸的天冬氨酸蛋白水解酶(cysteinyl aspartate specific proteinase,caspase-9)的蛋白表达;采用流式细胞仪(flow cytometry,FCM)检测USMCs细胞凋亡率,采用噻唑蓝[3-(4, 5-dimethyl-2-thiazolyl)-2, 5-diphenyl-2-H-tetrazolium bromide,MTT]检测尿USMCs细胞增殖率。结果:成功建立SD大鼠SUI模型。HE染色和Masson染色显示:与SUI组比较,DFMG组尿道括约肌病理症状显著改善,MBC,LPP和ALPP均有显著提高(均P<0.05)。RT-PCR结果显示:与SUI组比较,DFMG组USMCs细胞miR-26b mRNA表达升高(P<0.05),PENT mRNA表达下调(P<0.05)。Western印迹显示:与SUI组比较,DFMG组PENT,Bax,Cyt-C和caspase-3蛋白均下调(均P<0.05);而PI3K,AKT和Bcl-2蛋白表达均上调(均P<0.05);并伴随USMCs细胞凋亡率降低(P<0.05),增殖率增高(P<0.05)。结论:DFMG可以显著改善SUI模型鼠尿动力学症状,其机制可能与上调miR-26b表达、调控PI3/AKT-Bcl-2/Bax信号通路而抑制细胞凋亡有关。. Topics: Animals; Apoptosis; bcl-2-Associated X Protein; Caspase 3; Cell Proliferation; Cytochromes c; Disease Models, Animal; Genistein; Male; MicroRNAs; Phosphatidylinositol 3-Kinases; Proto-Oncogene Proteins c-akt; Proto-Oncogene Proteins c-bcl-2; Rats; Rats, Sprague-Dawley; Up-Regulation; Urethra; Urinary Bladder; Urinary Incontinence, Stress | 2018 |