cytochrome-c-t and Peroxisomal-Disorders

Peroxisomes cooperate with mitochondria in the performance of cellular metabolic functions, such as fatty acid oxidation and the maintenance of redox homeostasis. However, whether peroxisomes also regulate mitochondrial fission-fusion dynamics or mitochondrion-dependent apoptosis remained unclear. We now show that genetic ablation of the peroxins Pex3 or Pex5, which are essential for peroxisome biogenesis, results in mitochondrial fragmentation in mouse embryonic fibroblasts (MEFs) in a manner dependent on Drp1 (also known as DNM1L). Conversely, treatment with 4-PBA, which results in peroxisome proliferation, resulted in mitochondrial elongation in wild-type MEFs, but not in Pex3-knockout MEFs. We further found that peroxisome deficiency increased the levels of cytosolic cytochrome

Topics: Animals; Apoptosis; Butylamines; Caspases; Cell Line; Cytochromes c; Dynamins; Humans; Lipoproteins; Membrane Proteins; Mice; Mice, Inbred C57BL; Mitochondria; Mitochondrial Dynamics; Peroxins; Peroxisomal Disorders; Peroxisome-Targeting Signal 1 Receptor; Peroxisomes; RNA Interference; RNA, Small Interfering