cytochrome-c-t and Periodontitis

The aim of this study done in patients with periodontitis was (a) to determine superoxide anion production in whole blood by measuring cytochrome c reduction; (b) to assess the effects of oxidative burst in periodontal disease by analyzing lipid peroxidation and degradation of DNA bases in the peripheral and gingival blood; (c) to search for associations between superoxide anion production, lipid peroxidation, 8-hydroxy-2-deoxyguanosine concentration and clinical parameters. Total antioxidant status in peripheral and gingival serum was also studied and correlated with periodontal clinical status. The effects of some risk factors of periodontitis on oxidative stress were analyzed. The study group included 56 patients with untreated periodontitis. The control group consisted of 25 healthy volunteers without any pathological changes in the periodontium. The most important findings of the study are: (a) negative correlation between cytochrome c reduction and periodontal disease index in the study group (p=0.026); (b) positive correlation between anti-ox-LDL autoantibody titres in gingival blood and 8-OHdG levels in venous blood (p<0.001); (c) negative correlation between 8-OHdG concentration in venous blood and total antioxidant status (TAS) in gingival blood serum in patients and positive correlation in controls; (d) significantly higher levels of 8-OHdG in gingival blood in each subgroup of patients as compared with controls; (e) significantly lower TAS in venous blood serum in each subgroup as compared with controls; (f) negative correlation between 8-OHdG concentration in gingival blood and TAS in venous blood serum (p=0.028). Oxidative stress in periodontitis expressed by elevated concentrations of ROS and accompanied by suppressed antioxidant activity in gingival blood may accelerate lesion formation in periodontal tissues.

Topics: 8-Hydroxy-2'-Deoxyguanosine; Adult; Antioxidants; Cytochromes c; Deoxyguanosine; Female; Gingiva; Humans; Lipid Peroxidation; Male; Middle Aged; Periodontitis; Reactive Oxygen Species

The aim of the study was to estimate the influence of tobacco smoking and the clinical type of periodontitis on the chosen parameters of oxidative stress (superoxide anion generation on the ground of cytochrome c reduction in the whole blood, the concentration of autoantibodies for oxidized low-density lipoprotein in venous and gingival blood, the level of 8-hydroxy-2-deoxyguanosine (8-OHdG) as a marker of oxidative DNA damage in venous and gingival blood and the total antioxidant capacity in venous and gingival blood).. The study included 50 periodontitis patients (20 patients with generalized aggressive periodontitis - GAP and 30 patients with chronic periodontitis--CP), aged 28-55 (the mean value 40.3). There were 28 females and 22 males. The control group consisted of 25 volunteers in good general health, aged 2-50 (the mean value 37.8). There were 15 women in this group. In clinical examination ofperiodontium following indexes were included: plaque index (Silness and Löe), aproximal plaque index (Lange et al.), bleeding upon probing (Saxer and Mühlemann), pockets depth, the number of teeth.. Significant influence of periodontal diagnosis on the level of 8-OHdG in gingival blood and total antioxidant capacity and also all analyzed clinical parameters was revealed. Significant total influence ofperiodontal diagnosis with tobacco smoking on total antioxidant capacity was also seen in venous blood (p = 0.004).. 1) periodontal status impacted in significant way on the level of 8-OHdG in gingival blood and total antioxidant capacity in venous blood, 2) tobacco smoking and chronic periodontitis presence were connected with significantly decreasing levels of total antioxidant capacity in venous blood; this fact may be under importance in connection between periodontitis and atherosclerosis, 3) tobacco smoking was a significant factor in the progression ofperiodontitis (higher levels of plaque indexes, deeper periodontal pockets in nicotine addicted patients).

Topics: 8-Hydroxy-2'-Deoxyguanosine; Adult; Antioxidants; Biomarkers; Cytochromes c; Deoxyguanosine; Female; Gingiva; Humans; Lipid Peroxidation; Male; Middle Aged; Oxidative Stress; Periodontitis; Reactive Oxygen Species; Smoking; Tobacco Use Disorder

We studied morphological characteristics of the regenerative process in gingival tissues during therapy of chronic periodontitis. Energostim stimulated macrophageal reaction and promoted neoangiogenesis in the inflammatory infiltrate. It was not observed after traditional drug therapy. Energostim promoted vascularization in regenerating tissues, normalized the structure at a greater area of the lamina propria of the gingiva, and prevented fibrous and sclerotic changes. The volume of histiolymphocytic infiltrates in regenerating gingival tissues decreased after application of intradental splints.

Topics: Capillaries; Chronic Disease; Cytochromes c; Drug Combinations; Epithelium; Fibroblasts; Gingiva; Gingivitis; Humans; Inflammation; Inosine; Macrophages; Mucous Membrane; NAD; Periodontitis; Splints; Time Factors; Wound Healing