cytochrome-c-t and Hearing-Loss--Sensorineural

Our findings demonstrated that purple bamboo salt (PBS)-pharmaceutical acupuncture has an ameliorative effect on cisplatin-induced ototoxicity.. We have previously reported that PBS exhibited anti-allergic and anti-inflammatory actions in vitro and in vivo. Pharmaceutical acupuncture is a traditional oriental therapeutic technique that combines acupuncture with herbal treatment. The aim of this study was to investigate the protective effect and mechanism of PBS-pharmaceutical acupuncture against cisplatin-induced ototoxicity in the auditory cell line, HEI-OC1, and in vivo.. The ELISA method, a caspase-3 assay, an MTT assay, Western blot analysis, and a luciferase assay were utilized to investigate the effect of PBS in vivo and in vitro.. When it was acupunctured at the Ermen acupoint (triple energizer meridian 21) after an administration of cisplatin, PBS-pharmaceutical acupuncture significantly suppressed interleukin (IL)-6 production and caspase-3 activation induced by cisplatin in the cochlea. In addition, PBS significantly inhibited cisplatin-induced apoptosis and IL-6 production in HEI-OC1 cells. PBS also suppressed cytochrome c release and caspase-3 activation, and it inhibited extracellular signal-related kinase and nuclear factor-κB activation in HEI-OC1 cells.

Topics: Acupuncture Points; Acupuncture, Ear; Animals; Anti-Inflammatory Agents; Antineoplastic Agents; Apoptosis; Caspase 3; Cell Line; Cisplatin; Cytochromes c; Cytokines; Extracellular Signal-Regulated MAP Kinases; Hair Cells, Auditory, Outer; Hearing Loss, Sensorineural; Interleukin-6; Male; Medicine, Korean Traditional; Mice; Mice, Inbred C57BL; NF-kappa B; Phytotherapy; Plant Extracts; Powders; Sasa

The results reported here provide the first evidence of the production of superoxide, a biologically relevant reactive oxygen species (ROS), in human inner ear perilymph (hIP) in pathological conditions, by the activity of the xanthine dehydrogenase/xanthine oxidase (XA/XO) enzyme system.. To investigate the presence of ROS in hIP.. Since damage and apoptosis of inner ear hair cells may occur as a result of ROS-mediated injury, we investigated the presence and production of ROS in 105 hIP samples; 98 collected from patients affected by profound sensorineural hearing loss, during surgery for cochlear implantation, and 7 controls, collected from patients affected by otosclerosis, in case of spontaneous leakage after stapedotomy. ROS production was investigated by spectrophotometric analysis and polyacrylamide gel electrophoresis (SDS-PAGE).. In hIP samples tested by cytochrome c reduction kinetics, the average superoxide production was 27.34 mumoles per mg of total protein, against 0.36 in controls. Some of these hIP samples, analyzed by cytochrome c reduction kinetics in the presence of xanthine, were found to be positive for ROS-producing XA/XO enzyme. These results were supported by SDS-PAGE analysis.

Topics: Adolescent; Adult; Aged; Child; Child, Preschool; Cochlear Implants; Cytochromes c; Ear, Inner; Electrophoresis, Polyacrylamide Gel; Female; Hearing Loss, Sensorineural; Humans; Infant; Infant, Newborn; Male; Middle Aged; Otosclerosis; Perilymph; Reactive Oxygen Species; Spectrophotometry; Stapes Surgery; Uric Acid; Young Adult