cytochrome-c-t has been researched along with Colitis* in 2 studies
2 other study(ies) available for cytochrome-c-t and Colitis
| Article | Year |
|---|---|
Chlorogenic acid ameliorates intestinal mitochondrial injury by increasing antioxidant effects and activity of respiratory complexes.
Dietary polyphenols are thought to be beneficial for human health by acting as antioxidants. Chlorogenic acid (CGA) is abundant in plant-based foods as an ester of caffeic acid and quinic acid. In this study, we investigated the effects of CGA on mitochondrial protection. Our results demonstrated that pretreatment with CGA ameliorated the intestinal mitochondrial injury induced by H2O2; membrane potential was increased, mitochondrial swelling, levels of reactive oxygen species, contents of 8-hydroxy-2-deoxyguanosine, and cytochrome c released were decreased. The beneficial effects of CGA were accompanied by an increase in antioxidant and respiratory-chain complex I, IV, and V activities. In trinitrobenzene-sulfonic acid-induced colitic rats indicated that CGA supplementation improved mitochondria ultrastructure and decreased mitochondrial injury. Our results suggest a promising role for CGA as a mitochondria-targeted antioxidant in combating intestinal oxidative injury. Daily intake of diets containing CGA, such as coffee and honeysuckle, may be useful for prevention of intestinal diseases. Topics: Animals; Antioxidants; Chlorogenic Acid; Colitis; Cytochromes c; Electron Transport Complex I; Electron Transport Complex IV; Enzyme Activation; Female; Glutathione; Hydrogen Peroxide; Membrane Potential, Mitochondrial; Mitochondria; Mitochondrial Swelling; Oxidation-Reduction; Oxidative Stress; Rats; Rats, Sprague-Dawley; Reactive Oxygen Species; Trinitrobenzenesulfonic Acid | 2016 |
Apoptosis of human colorectal carcinoma cells is induced by blocking hepatoma-derived growth factor.
Hepatoma-derived growth factor (HDGF) is a novel multifunctional growth factor that elicits pleiotropic effects on biological processes such as lung remodeling and renal development. Recent studies demonstrated that HDGF is related to tumor proliferation, invasion, angiogenesis, and apoptosis. However, the molecular mechanism of HDGF's involvement in apoptosis remains to be clarified. In this study, we first analyze the role of HDGF in colorectal carcinoma (CRC) progression by immunohistochemistry. Then we used small interference RNA (HDGF-siRNA) to block HDGF and assessed its effect on inducing apoptosis of CRC loVo cells. Apoptosis was detected using flow cytometry (FCM), DNA ladder analysis, and Hoechst 33258 staining. In addition, the expression levels of some apoptosis-related proteins were examined by western blot. The result showed that HDGF expression gradually increased in the colorectal carcinogenesis process. Further studies demonstrated that knock-down of HDGF can down-regulate the survivin, activate the mitochondrial pathway, and induce apoptosis in loVo cells. These findings suggest that HDGF is involved in colorectal carcinogenesis process. Further blocking HDGF exhibits potent pro-apoptotic properties in colon cancer cells. Thus, HDGF might be a potential therapeutic target for human colorectal cancer. These findings may have major implications in the treatment of colorectal cancer. Topics: Adenoma; Apoptosis; Blotting, Western; Cell Proliferation; Colitis; Colon; Colorectal Neoplasms; Cytochromes c; Female; Flow Cytometry; Humans; Immunoenzyme Techniques; Intercellular Signaling Peptides and Proteins; Lymphatic Metastasis; Male; Middle Aged; Neoplasm Invasiveness; Precancerous Conditions; Rectum; Reverse Transcriptase Polymerase Chain Reaction; RNA, Messenger; RNA, Small Interfering | 2010 |