cytochalasin-d has been researched along with Nerve-Degeneration* in 1 studies
1 other study(ies) available for cytochalasin-d and Nerve-Degeneration
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GTPase RhoB: an early predictor of neuronal death after transient focal ischemia in mice.
Applying the recently developed DNA array technique to a murine stroke model, we found that the gene coding for RhoB, a member of the family of GTPases that regulate a variety of signal transduction pathways, is upregulated in ischemia-damaged neurons. RhoB immunoreactivity precedes DNA single-strand breaks and heralds the evolving infarct, making it an early predictor of neuronal death. Expression of RhoB colocalized with drastic rearrangement of the actin cytoarchitecture indicates a role for Rho in postischemic morphological changes. Apoptosis in a murine hippocampal cell line was also associated with an early increase in RhoB protein. Activation of caspase-3, a crucial step in apoptosis, could be inhibited by cytochalasin D, a substance that counteracts the actin-modulating activity of Rho GTPases, indicating that Rho proteins may have impact on injury-initiated neuronal signal transduction. Our findings make Rho GTPases potential targets for the development of drugs aimed at limiting neuronal death following brain damage. Topics: Actin Cytoskeleton; Animals; Apoptosis; Brain Infarction; Brain Ischemia; Caspase 3; Caspases; Cells, Cultured; Cytochalasin D; Disease Models, Animal; DNA Damage; DNA, Single-Stranded; Gene Expression; Hippocampus; Immunohistochemistry; Mice; Mice, Inbred C57BL; Nerve Degeneration; Neurons; Oligonucleotide Array Sequence Analysis; Predictive Value of Tests; Reperfusion Injury; rhoB GTP-Binding Protein; RNA, Messenger; Time Factors; Up-Regulation | 2001 |