cytochalasin-b has been researched along with Urethritis* in 3 studies
3 other study(ies) available for cytochalasin-b and Urethritis
| Article | Year |
|---|---|
[Isolation of Chlamydia trachomatis on Hela 229 cells treated with cytochalasin B in 330 cases of lower genital infections in men and women].
A technique of isolating Chlamydia trachomatis on Hela 229 cells treated with cytochalasine B after the inoculation is described. By this technique, Chlamydia trachomatis was isolated from 36,6% of 186 men with non-gonococcal urethritis and from 20,8% of 114 women with signs of cervicitis. Topics: Adult; Bacteriological Techniques; Chlamydia Infections; Chlamydia trachomatis; Cytochalasin B; Female; HeLa Cells; Humans; Male; Middle Aged; Urethritis; Uterine Cervicitis | 1983 |
Comparison of various McCoy cell treatment procedures used for detection of Chlamydia trachomatis.
McCoy cells treated in six different ways, in addition to untreated cells, were compared to determine which gave rise to the largest number of Chlamydia trachomatis inclusions when tested with a laboratory-passaged strain. The same batch of cells was treated by irradiation, preinoculation exposure to cytochalasin B or 5-iodo-2'-deoxyuridine, and postinoculation exposure to cycloheximide, hydrocortisone, or emetine. Significantly more inclusions were always found in cells which had been treated with cycloheximide than in cells treated in any other way. Conversely, untreated McCoy cells always had significantly fewer inclusions than cells which had received some form of treatment. Similar results were obtained when cycloheximide-treated, irradiated, and untreated cells were inoculated with urethral specimens containing unpassaged organisms. Topics: Bacteriological Techniques; Cells, Cultured; Chlamydia Infections; Chlamydia trachomatis; Cobalt Radioisotopes; Cycloheximide; Cytochalasin B; Emetine; Humans; Hydrocortisone; Idoxuridine; Male; Urethritis | 1979 |
[New "chlamydia" isolation methods applied to the current medical practice and epidemiology (author's transl)].
From 935 men attending a consultation for veneral diseases and 117 women suffering from vaginal discharge, 1 052 genito urinary tract specimens were inoculated on monolayers of McCoy cells which were irradiated or treated with either cytochalasin B or 5-iodo-2-deoxyuridine. The value of these technics was estimated in routine diagnostic procedures according to the number of positive cultures, regardless of the number of inclusions per culture and also by comparison of the number of inclusions seen in cells inoculated with reference strains of lymphogranuloma venereum, trachoma and human urethral Chlamydia. The best method seems to consist of the use of McCoy cells treated with cytochalasin B for routine isolation and of acridine orange for the staining of inclusions. Topics: Chlamydia; Chlamydia Infections; Cytochalasin B; DEAE-Dextran; Female; Humans; Idoxuridine; Leukorrhea; Male; Urethritis; Uterine Cervicitis | 1978 |