cytochalasin-b and Precursor-T-Cell-Lymphoblastic-Leukemia-Lymphoma

Glaucumolides A (1) and B (2), novel biscembranes composed of an unprecedented α,β-unsaturated ε-lactone, along with the known metabolites ximaolide A (3) and isosarcophytonolide D (4), were isolated from the cultured soft coral Sarcophyton glaucum. The structures of the new metabolites were determined by extensive spectroscopic analyses. Compounds 1 and 2 were shown to exhibit cytotoxicity against a limited panel of cancer cell lines. In anti-inflammation assay, compounds 1 and 2 displayed strong inhibition of superoxide anion generation and elastase release in human neutrophils stimulated by fMLP/CB. Furthermore, both 1 and 2 were shown to significantly inhibit the accumulation of the pro-inflammatory inducible nitric oxide synthase protein, and compounds 1-3 were found to effectively reduce the expression of cyclooxygenase-2 protein, in lipopolysaccharide-stimulated RAW264.7 macrophage cells.

Topics: Animals; Anthozoa; Anti-Inflammatory Agents; Antineoplastic Agents; Cell Line, Tumor; Cyclooxygenase 2; Cytochalasin B; Diterpenes; Drug Screening Assays, Antitumor; HL-60 Cells; Humans; Inflammation; Lactones; Leukemia, Promyelocytic, Acute; Lipopolysaccharides; Macrophages; Mice; Neutrophils; Nitric Oxide Synthase Type II; Precursor T-Cell Lymphoblastic Leukemia-Lymphoma; Superoxides