cytochalasin-b and Kidney-Neoplasms

Topics: Carcinoma, Renal Cell; Cell Survival; Cobalt Radioisotopes; Cytochalasin B; Gamma Rays; Humans; In Vitro Techniques; Kidney Neoplasms; Micronucleus Tests; Tumor Cells, Cultured

The correlation between cell survival curve and dose response curve of MN frequency following irradiations was studied using cytokinesis-block method. Both dose response curves were analyzed by linear quadratic model, i.e. SF = exp (- alpha D - beta D2) and MN frequency = aD + bD2 + c. A good correlation between alpha/beta and a/b ratios was observed in repeated paired experiments (gamma = 0.97). When the cells were treated with BUdR, alpha-type radiosensitizer, a value in dose response curve of MN frequency increased but b value did not. In 10 renal cell carcinomas, the linear correlations between cell surviving fractions and MN frequencies were observed. When radioresponses of 5 esophageal cancer cell lines were evaluated with MN assay, wide range of a/b ratios was found. These data present that MN frequency assay using CB method is available as a tool of rapid assay of radiosensitivity of cells.

Topics: Animals; Carcinoma, Renal Cell; Carcinoma, Squamous Cell; Cell Survival; Cytochalasin B; Dose-Response Relationship, Radiation; Esophageal Neoplasms; Humans; Kidney Neoplasms; Mice; Micronucleus Tests; Predictive Value of Tests; Radiation Tolerance; Tumor Cells, Cultured

Topics: Animals; Bromodeoxyuridine; Bucladesine; Cell Line; Cell Transformation, Neoplastic; Cells; Cells, Cultured; Cytochalasin B; Deoxyglucose; Dimethyl Sulfoxide; Dimethylformamide; Kidney Neoplasms; Male; Membranes; Mice; Mice, Inbred C3H; Neoplasms, Experimental; Theophylline