cytochalasin-b and Chronic-Disease

cytochalasin-b has been researched along with Chronic-Disease* in 3 studies

Other Studies

3 other study(ies) available for cytochalasin-b and Chronic-Disease

ArticleYear
In vitro release of elastase from human blood and gingival crevicular neutrophils.
    Archives of oral biology, 1991, Volume: 36, Issue:7

    Peripheral PMNs were collected from blood, and crevicular PMNs separated by filtration from gingival washings in 13 patients, aged 22-75 y, who had varying degrees of gingivitis and periodontitis. After pre-incubation with cytochalasin B, the same number of crevicular and peripheral cells were incubated either in PBS (with Ca2+ and Mg2+) (spontaneous release) or in the same buffer containing increasing concentrations of FMLP (stimulated release); elastase activity was measured in the supernatant by a fluorometric technique. There was a higher spontaneous release of enzyme from crevicular than from peripheral neutrophils. The average elastase activity in the supernatant of 1 x 10(4) crevicular cells was more than five times higher than that obtained from the same number of peripheral cells. However, stimulated crevicular PMNs liberated smaller amounts of enzyme than did stimulated peripheral PMNs. These results suggest that crevicular PMNs are already releasing elastase, and are consistent with the possibility that lysosomal enzymes contribute to tissue damage during gingivitis and periodontitis.

    Topics: Adult; Aged; Blood; Chronic Disease; Cytochalasin B; Gingival Crevicular Fluid; Gingival Pocket; Gingivitis; Humans; Middle Aged; N-Formylmethionine Leucyl-Phenylalanine; Neutrophils; Pancreatic Elastase; Periodontal Index; Periodontal Pocket; Periodontitis

1991
Chronic hyperglycemia increases the density of glucose transporters in human erythrocyte membranes.
    The Journal of clinical endocrinology and metabolism, 1991, Volume: 72, Issue:4

    We investigated the effect of chronic hyperglycemia on glucose transporters in erythrocytes of subjects with and without diabetes mellitus. We found a 22% increase in D-glucose-displaceable cytochalasin-B binding in erythrocyte membranes of diabetic subjects over those of controls (311 +/- 13 vs. 254 +/- 8 pmol/mg protein; P less than 0.001). This increased binding was due to a higher density of binding sites without a significant change in binding affinity. Cytochalasin-B binding to erythrocyte membrane correlated positively with both erythrocyte glycohemoglobin and serum glucose levels, but not with plasma C-peptide levels. The data are compatible with up-regulation of glucose transporters in the erythrocytes of subjects with chronic hyperglycemia. We suspect that this is brought about by increased synthesis and membrane incorporation of the glucose transporter during erythropoiesis.

    Topics: Adult; Aged; Blood Glucose; C-Peptide; Chronic Disease; Cytochalasin B; Diabetes Mellitus; Erythrocyte Membrane; Female; Glycated Hemoglobin; Humans; Hyperglycemia; Male; Middle Aged; Monosaccharide Transport Proteins; Reference Values

1991
The specificity of IgG- and IgM-class smooth muscle antibody in the sera of patients with multiple sclerosis and active chronic hepatitis.
    Clinical immunology and immunopathology, 1979, Volume: 14, Issue:2

    Topics: Animals; Antibody Specificity; Chronic Disease; Colchicine; Cytochalasin B; Embryo, Mammalian; Epithelium; Female; Fibroblasts; Fluorescent Antibody Technique; Hepatitis; Humans; Immunoglobulin G; Immunoglobulin M; Lung; Multiple Sclerosis; Muscle, Smooth; Pregnancy; Rats; Vinblastine

1979