cytellin and Psoriasis

Topics: Animals; Inflammation; Mice; Molecular Docking Simulation; Phytosterols; Psoriasis; Sitosterols; Stigmasterol; Swine

Andrographis nallamalayana is being widely used as tribal medicine in the treatment of leucoderma and mouth ulcers. Chemical profiling of methanolic extract of the whole plant (PE), using GC-MS and LC-MS, revealed the presence of compounds viz. α-tocopherol, β-sitosterol, tetradecanoic acid, monostearin, flavones/flavanones and their glycosides, chromones, etc. Topical application of imiquimod on the dorsal portion of male BALB/C mice resulted in the development of psoriatic symptoms (erythema, scaling, thickening and folding) with a mean disease activity index (DAI) of >7.0. Topical treatment with 100-μL PE (~6.4%/12.8%) formulations, for 12-days, resulted in the alleviation of disease symptoms. Compared to water-based formulations, emu oil-based formulation, PE400EO was found more effective in reducing the mean DAI (>84%), keratinocyte count (>65%) (p < 0.01) and interleukin-22 (~70%) (p < 0.05). We report, for the first time, anti-psoriatic activity of A. nallamalayana having great potential in developing a potent phytomedicine against psoriasis.

Topics: alpha-Tocopherol; Andrographis; Animals; Flavanones; Flavones; Gas Chromatography-Mass Spectrometry; Glycosides; Interleukin-22; Interleukins; Male; Methanol; Mice; Mice, Inbred BALB C; Plant Extracts; Psoriasis; Sitosterols

Topics: Cholesterol; Electrophoresis; Humans; Leadership; Psoriasis; Sitosterols; Steroids