cyproterone and Disease-Models--Animal

Mongolian gerbils (Meriones unguiculatus) were grouped into two experimental groups: GEx.01 suffered orchiectomy and after 30 days received doses of testosterone cipionate (T), while GEx.02 received weekly and alternated doses of the anti-androgens cyproterone acetate and flutamide for 30 days, and the animals were then euthanized. Structural evaluation reveals a more intense reduction in epithelial height in GEx.02. Smooth muscle cells (SMC) presented a star-shaped aspect after 30 days of hormonal ablation and basal membrane was shown to be more intensely grooved in GEx.01. In both groups, after hormonal replacement, recovery in epithelial height could be noted and the SMC presented its phenotypes, but an increase in RER was seen, characterizing a modulation from its contractile to secreting phenotype. In conclusion, the prostate presented involution capacity after androgen ablation and the ability to reorganize after hormonal replacement, but events resulting from orchiectomy and subsequent T replacement were shown to be more aggressive to the prostate.

Topics: Acid Phosphatase; Androgen Antagonists; Animals; Cyproterone; Disease Models, Animal; Endoplasmic Reticulum, Rough; Endothelium; Flutamide; Gerbillinae; Immunohistochemistry; Male; Microscopy, Electron, Transmission; Orchiectomy; Organ Size; Prostate; Prostatic Hyperplasia; Protein Tyrosine Phosphatases; Receptors, Androgen; Testosterone

We studied the prostates of 22 beagle dogs by light morphometric analysis under defined hormonal influences. Prostatic weight increased with 3 alpha-androstanediol (3 alpha-diol) alone and in combination with 17 beta-estradiol (E2). Two different prostatic hyperplasia models were established. 3 alpha-Diol brings about diffuse glandular prostatic hyperplasia with an absolute increase of glandular parenchyma and, together with E2, provokes stromal hyperplasia with squamous metaplasia of the epithelium and secondary cyst formation. In both models, the antiandrogen cyproterone acetate causes a significant reduction of the absolute and relative volumes of the glandular compartment with antiandrogenic effects on the stroma.

Topics: Androstane-3,17-diol; Androstanols; Animals; Castration; Cyproterone; Disease Models, Animal; Dogs; Estradiol; Male; Metaplasia; Prostate; Prostatic Hyperplasia; Stereoisomerism

The effect of 3 alpha-androstanediol (3 alpha-diol), 17 beta-estradiol (E2) and cyproterone acetate (CA) on prostates in castrated beagle dogs were investigated by histological and histochemical examinations. A significant increase of prostatic weight occurred after 6 months' treatment with 3 alpha-diol alone and in combination with E2. Histologically and histochemically, two different types of prostatic enlargement were observed: first, administration of 3 alpha-diol resulted in diffuse glandular hyperplasia with replacement of functional activity monitored by strongly positive reactions for acid phosphatase, aminopeptidase and zinc. Second, 3 alpha-diol plus E2 produced stratified squamous metaplasia with cystic lumina and loss of the typical morphological structure. These glands showed negative reactions for acid phosphatase, aminopeptidase and zinc. In both types of prostatic hyperplasia CA abolished epithelial or metaplastic proliferation and induced atrophy of glandular epithelium. In estrogenized dogs activation of the fibromuscular stroma was obvious. CA prevented prostatic hyperplasia by atrophying epithelial effects.

Topics: Acid Phosphatase; Aminopeptidases; Androstanols; Animals; Castration; Cyproterone; Cyproterone Acetate; Disease Models, Animal; Dogs; Estradiol; Male; Prostatic Hyperplasia; Zinc

Dog prostates under defined hormonal influences were investigated by light microscopy (LM), transmission electron microscopy (TEM) and scanning electron microscopy (SEM). The normal acinar cell apices exhibited well-developed, densely packed microvilli. TEM demonstrated numerous secretory granules, indicating a high secretory activity. Following castration, the acinar cells of the dog prostate showed rounded apices. Most of the microvilli disappeared and only knob-like protrusions were seen. Castrated dogs following administration of 3 alpha-androstandiol showed fewer microvilli than the controls; however, a well-developed undulating surface could be observed. Castrated dogs given 3 alpha-androstandiol and cyproterone acetate showed almost completely atrophic glandular cells. Prostates of dogs with spontaneous benign prostatic hyperplasia (BPH) were characterized by pleomorphism of cell shapes and surfaces. The different hormonal influences induced changes of the prostatic surface structures and secretory activity.

Topics: Androstanols; Animals; Castration; Cyproterone; Cytoplasmic Granules; Disease Models, Animal; Dogs; Drug Interactions; Estradiol; Gonadal Steroid Hormones; Male; Microscopy, Electron; Microscopy, Electron, Scanning; Prostate; Prostatic Hyperplasia

Topics: Androgen Antagonists; Animals; Cyproterone; Disease Models, Animal; Dose-Response Relationship, Drug; Humans; Male; Organ Size; Prostate; Prostatic Hyperplasia; Prostatic Neoplasms; Testosterone; Transplantation, Heterologous

Topics: Androstenedione; Animals; Cricetinae; Cyproterone; Disease Models, Animal; Disorders of Sex Development; Dogs; Erectile Dysfunction; Estradiol; Female; Guinea Pigs; Haplorhini; Homosexuality; Humans; Male; Posture; Rats; Sexual Abstinence; Sexual Behavior; Sexual Behavior, Animal; Sexual Dysfunction, Physiological; Species Specificity; Testosterone

Topics: Androgen Antagonists; Animals; Arginase; Body Weight; Castration; Cyclophosphamide; Cyproterone; Disease Models, Animal; DNA; Dogs; Drug Evaluation, Preclinical; Fluorouracil; Humans; Male; Organ Size; Oxidoreductases; Prostate; Prostatic Neoplasms; Rats