cycloaspeptide-a and Leukemia-P388

A new cyclic nitropeptide, psychrophilin D (1), together with two known cyclic peptides, cycloaspeptide A (2) and cycloaspeptide D (3), were isolated from the psychrotolerant fungus Penicillium algidum using C18 flash chromatography, LH-20 Sephadex and preparative HPLC. The structure of psychrophilin D (1) was derived from mass spectrometric information, 1D and 2D NMR spectra and Marfey's method. The compounds were tested in antimicrobial, antiviral, anticancer and antiplasmodial assays. Psychrophilin D (1) exhibited a moderate activity (ID50 10.1 microg/ml) in the P388 murine leukaemia cell assay. Cycloaspeptide A (2) and D (3) exhibited moderate activity (IC50 3.5 and 4.7 microg/ml, respectively) against Plasmodium falciparum.

Topics: Animals; Anti-Bacterial Agents; Antibiotics, Antineoplastic; Antimalarials; Antiviral Agents; Bacteria; Cell Line, Tumor; Chromatography, High Pressure Liquid; Circular Dichroism; Drug Screening Assays, Antitumor; Humans; Leukemia P388; Magnetic Resonance Spectroscopy; Mass Spectrometry; Mice; Microbial Sensitivity Tests; Penicillium; Peptides, Cyclic; Plasmodium falciparum; Viruses