cyclin-d1 and Pseudomonas-Infections

Contact lens were and eyelid suturing diminish overall Bcl-2 expression in the corneal epithelium, yet nuclear Bcl-2 in the exposed surface epithelial cells seems to be retained for longer periods when compared with the retention in control eyes. This observation may explain why there is a decrease in surface cell exfoliation during CTL wear, because nuclear Bcl-2 loss needs to precede apoptotic surface cell exfoliation. In addition, if the hypothesis is true that Bcl-2 also has a regulatory function in the proliferation and differentiation of tissues, the overall reduced expression of Bcl-2 (nuclear plus cytoplasmic) may clarify the decrease in proliferation of the corneal epithelium seen during CTL wear. Future studies should clarify the role of Bcl-2 in the homeostatic dynamics of the corneal epithelium. CTL wear and eyelid suturing provide excellent noninflammatory models to advance knowledge of Bcl-2 and its regulatory roles. Additionally, these models should help researchers gain a better understanding of the CTL-associated effects on corneal epithelium.

Topics: Animals; Cell Death; Cell Differentiation; Cell Division; Cell Survival; Contact Lenses; Corneal Ulcer; Cyclin D1; Epithelium, Corneal; Homeostasis; Humans; Keratitis; Oxygen; Permeability; Pseudomonas Infections

Salvia officinalis L. (Lamiaceae) is known to have antibacterial properties possibly conducive to the healing process of infected wounds.. The present study aimed to evaluate the effects of an ointment containing Salvia officinalis essential oil (SOO) on an infected wound model.. Essential oil hydrodistillated from the dried leaves of the plant was analyzed by GC-FID and GC-MS. After creating two full-thickness cutaneous wounds, mice were classified into four groups, control, and animals treated with 2 % mupirocin® (standard positive drug), and 2 % and 4 % (w/w) of SOO. In order to evaluate the effects of SOO on the wound healing phases, the expression levels of interleukin-6 (IL-6), interleukin-1β (IL-1β), tumor necrosis factor-α (TNF-α), cyclin-D1, Bcl-2, fibroblast growth factor-2 (FGF-2) and vascular endothelial growth factors (VEGF) were analyzed using qRT-PCR. Immunohistochemistry analysis, tissue total antioxidant capacity (TAC) and malondialdehyde (MDA) were further assessed in all groups.. Concerning essential oil, the main compounds were found to be cis-thujone (26.8 %), camphor (16.4 %), trans-thujone (14.1 %) and 1,8-cineole (10.8 %). Our findings showed that the topical application of SOO was able to shorten the inflammatory phase and accelerate the cellular proliferation, re-vascularization, collagen deposition and re-epithelialization in comparison to the control group (p < 0.05). Moreover, increased mRNA levels of FGF-2 and VEGF, and up-regulation of cyclin-D1 and Bcl-2 were observed following the topical application of SOO compared to the control group (p < 0.05). The expression levels of IL-6, IL-1β and TNF-α were reduced in animals treated with SOO on days 3, 7 and 14 (p < 0.05).. Administration of SOO increased the TAC level and reduced the MDA content and levels of IL-1β and TNF-α. It is concluded that SOO is able to accelerate the wound healing process by regulating the expression of pro-inflammatory cytokines, growth factors, and antioxidant properties.

Topics: Administration, Cutaneous; Animals; Anti-Bacterial Agents; Cyclin D1; Cytokines; Disease Models, Animal; Fibroblast Growth Factor 2; Inflammation Mediators; Male; Mice, Inbred BALB C; Oils, Volatile; Plant Oils; Proto-Oncogene Proteins c-bcl-2; Pseudomonas Infections; Salvia officinalis; Staphylococcal Infections; Time Factors; Vascular Endothelial Growth Factor A; Wound Healing; Wound Infection