cyclin-d1 and Leukoplakia

The aim of this study was to determine, by immunohistochemical methods, the expression of nEGFR and markers of cell proliferation (Ki-67), cell cycle (mEGFR, p53, cyclin D1), and tumor stem cells (ABCG2) in 59 pathohistological samples of healthy oral mucosa, 50 oral premalignant changes (leukoplakia and erythroplakia), and 52 oral squamous cell carcinomas (OSCC). An increase in the expression of mEGFR and nEGFR was found with the development of the disease (

Topics: Carcinoma, Squamous Cell; Cyclin D1; ErbB Receptors; Head and Neck Neoplasms; Humans; Ki-67 Antigen; Leukoplakia; Mouth Neoplasms; Squamous Cell Carcinoma of Head and Neck; Tumor Suppressor Protein p53

EMS1 (chromosome eleven, band q13, mammary tumor and squamous cell carcinoma-associated gene 1) gene amplification and the concomitant cortactin overexpression have been reported to associate with poor prognosis and tumor metastasis. In this study, we examined cortactin expression by immunohistochemistry in human oral tumors and murine tongue tumors which were induced by the carcinogen 4-nitroquinoline 1-oxide (4-NQO). The immunostaining results show over- to moderate expression of cortactin in 85% (104/122) of oral squamous cell carcinoma (OSCC) tissues and in all 15 leukoplakia tissues examined. Further, statistical analysis indicates that cortactin overexpression appears to be a predictor for shorter survival and poorer prognosis in OSCC patients. In an animal model, cortactin is shown to upregulate in infiltrating squamous cell carcinoma, papilloma, and epithelia with squamous hyperplasia, indicating that cortactin induction is an early event during oral carcinogenesis. It is suggested that cortactin expression is mediated in the progression of pre-malignancy to papilloma, based on earlier cortactin induction in pre-malignancy preceding cyclin D1 in papilloma. In conclusion, cortactin overexpression is frequently observed in human OSCC and mouse tongue tumors. Thus, cortactin may have an important role in the development of oral tumors in human and mice. © 2016 Wiley Periodicals, Inc. Environ Toxicol 32: 799-812, 2017.

Topics: 4-Nitroquinoline-1-oxide; Adult; Animals; Areca; Carcinogenesis; Carcinoma, Squamous Cell; Cortactin; Cyclin D1; Disease Models, Animal; Disease-Free Survival; Female; Humans; Immunohistochemistry; Kaplan-Meier Estimate; Leukoplakia; Male; Mice; Mice, Inbred BALB C; Middle Aged; Mouth Neoplasms; Plant Extracts; Polymerase Chain Reaction; Prognosis; Proportional Hazards Models; Tongue Neoplasms; Up-Regulation