cyclin-d1 and Heart-Diseases

Our aim was to investigate the role of interleukin-6 (IL-6) in myocardial regeneration from mice after cardiac injury. The newborn mice were divided into the following 4 groups (16 in each group): sham group, model group, IL-6-/- group (IL-6 knockout) and IL-6 group (IL-6 overexpression). Electrocardiography was performed on all mice and found higher LVEDD, LVESD and IVST and lower LVEF and LVFS in the IL-6 group compared with the sham group. Using HE staining, severe myocardial injury combined with infarction and fibrosis were observed in the IL-6-/- group, while the damaged myocardial tissue was repaired to some extent in the IL-6 group. The expression of IL-6 in the IL-6 group were significantly up-regulated. BrdU immunofluorescence found that the IL-6-/- group had the least number of BrdU positive cells, while the IL-6 group had more BrdU positive cells than the model group and the IL-6-/- group. Expressions of IL-6, cyclinD1 and Bcl-2 in the IL-6 group were up-regulated compared with other groups. In conclusion, IL-6 overexpression could enhance cardiomyocyte proliferation and relevant protein expression in mice myocardium, thus promoting cardiac regeneration.

Topics: Animals; Animals, Newborn; Cell Proliferation; Cyclin D1; Disease Models, Animal; Heart Diseases; Interleukin-6; Mice, Knockout; Myocytes, Cardiac; Phosphorylation; Proto-Oncogene Proteins c-bcl-2; Regeneration; Signal Transduction; STAT3 Transcription Factor; Stroke Volume; Vascular Endothelial Growth Factor A; Ventricular Function, Left

Topics: Adenoviridae; Animals; Cell Cycle; Cell Differentiation; Cell Proliferation; Cyclin D1; Cyclin-Dependent Kinase 4; Gene Transfer Techniques; Genetic Therapy; Genetic Vectors; Heart Diseases; Humans; Myocardium; Regeneration; S-Phase Kinase-Associated Proteins; Time Factors; Ventricular Function, Left