cyclin-d1 and Ankylosis

To preliminarily investigate the temporal patterns of the endogenous mRNA expression for members of the Wnt signaling and a series of genes regulating bone formation during the development of traumatic temporomandibular joint (TMJ) bony ankylosis in a sheep model.. Six sheep were used for the induction of bony ankylosis of TMJ. We performed a condylar fracture, excision of the lateral 2/3 disc and serious injury to the glenoid fossa to induce bony ankylosis on the right TMJ. An isolated condylar fracture was performed on the left side. Two sheep were sacrificed at 1 month, 3 months, and 6 months after surgery, respectively. The specimens from the ankylosed joint and the condylar fracture were harvested for RNA extraction respectively. In this report (Part I), only the bony ankylosed samples were used for analysis of gene expressions. The specimens 1 month postoperatively were taken as the control, and the changes of expression of target genes over time were examined by real-time PCR.. mRNA expression of Wnt1, Wnt2b, Wnt3a, β-catenin, Sfrp1, Lrp6, Lef1, CyclinD1, and Runx2 was up-regulated at 3 and 6 months compared with 1 month. The expression of Wnt5a, Sox9, and Osterix was up-regulated with a peak at 3 months, and then fell back to the basal levels at 6 months. The expression of Ocn began to up-regulate until 6 month postoperatively.. Our findings suggested that Wnt signaling was involved in the formation of traumatic TMJ bony ankylosis and thus may be a potential therapeutic target for the treatment of the disease in the future.

Topics: Animals; Ankylosis; beta Catenin; Core Binding Factor Alpha 1 Subunit; Cyclin D1; Disease Models, Animal; Gene Expression Profiling; Glycoproteins; Intercellular Signaling Peptides and Proteins; Intracellular Signaling Peptides and Proteins; Low Density Lipoprotein Receptor-Related Protein-6; Lymphoid Enhancer-Binding Factor 1; Mandibular Condyle; Mandibular Fractures; Osteocalcin; Osteogenesis; Pilot Projects; Proto-Oncogene Proteins; Sheep; SOX9 Transcription Factor; Temporal Bone; Temporomandibular Joint; Temporomandibular Joint Disc; Temporomandibular Joint Disorders; Transcription Factors; Wnt Proteins; Wnt Signaling Pathway; Wnt1 Protein; Wnt3A Protein

The purpose of the study was to preliminarily explore the differential expressions of a series of genes regulating bone formation in temporomandibular joint (TMJ) fibrous ankylosis, bony ankylosis and condylar fracture healing.. The cDNA from either the bony ankylosed callus or fracture callus of the 6 sheep, as described in the part I, were both used in the study. The differences of gene expressions between bony ankylosis and condylar fracture at 1, 3, and 6 months postoperatively were measured by real-time PCR, with 2 samples at each time point. In addition, another 2 sheep were added to have fibrous ankylosis induced on the right TMJ, and 1 sheep was sacrificed at 3 and 6 months after surgery, respectively. The differences of gene expressions between fibrous and bony ankylosis at 3 and 6 months postoperatively were measured by real-time PCR.. Bony ankylosis showed higher mRNA expression trends in Wnt2b, Wnt5a, β-Catenin, Lef1, CyclinD1, Runx2, Osterix, Sox9, Col10a1, Alp, Ocn, Bmp2, and Bmp7 compared to fibrous ankylosis, although no statistical analysis was performed due to the very small sample size. Whereas bony ankylosis showed a significant lower expression of Wnt5a, β-Catenin, Lef1, Runx2, Osterix, Sox9, Col10a1, Alp, Ocn and Bmp4 compared to condylar fracture at several time points (P < 0.05).. Our data provided a preliminary molecular evidence for the hypothesis that the development of traumatic TMJ bony ankylosis was the course of delayed bone healing or hypertrophic nonunion, and deserved to be further studied.

Topics: Alkaline Phosphatase; Animals; Ankylosis; beta Catenin; Bone Morphogenetic Protein 2; Bone Morphogenetic Protein 7; Bony Callus; Collagen Type X; Core Binding Factor Alpha 1 Subunit; Cyclin D1; Disease Models, Animal; Fibrosis; Fracture Healing; Gene Expression Profiling; Gene Expression Regulation; Lymphoid Enhancer-Binding Factor 1; Mandibular Condyle; Mandibular Fractures; Osteocalcin; Pilot Projects; Proto-Oncogene Proteins; Sheep; SOX9 Transcription Factor; Temporomandibular Joint; Temporomandibular Joint Disorders; Transcription Factors; Wnt Proteins