cyclic-gmp and Wounds-and-Injuries

In order to investigate the correlation between traumatic servity and blood cAMP and cGMP levels in the patients with acute trauma and its clinical significance, 120 cases of trauma were randomly selected and divided into 4 groups (n = 30 in each group): mildly traumatic group (ISS < or = 9), moderately traumatic group (ISS = 10-16), severely traumatic group (ISS = 17-25) and dangerously traumatic group (> 25). The cAMP and cGMP levels were assayed in sera, leucocytes and platelets respectively in 6 h and 24 h after trauma. The results showed that cAMP and cGMP levels were elevated significantly in sera and platelets (P < 0.05 or P < 0.01), meanwhile cGMP levels in leucocytes (P < 0.05). It was concluded that cAMP and cGMP might play an important role in traumatic stress, participate in the cellular signal transducation and promote the immune function of leucocytes and the coagulation founction of platelets. Serum cAMP and cGMP levels were upregulated correspondingly as ISS increased, and positively correlated to the traumatic severity.

Topics: Adolescent; Adult; Blood Platelets; Cyclic AMP; Cyclic GMP; Female; Humans; Injury Severity Score; Leukocytes; Male; Middle Aged; Wounds and Injuries

The effect of collagen, its hydrolysate and glycin on metabolism of cyclic nucleotides in wound tissue was studied in experiments on animals. Collagen was found to reduce the cAMP level in muscles of the wound fundus, while the concentration of cGMP remained unchanged. Collagen hydrolysate induced unidirectional changes in cyclic nucleotides, whereas glycin opposite ones. The basal activity of cAMP phosphodiesterase was not changed. The mechanism of the stimulatory effect of collagen on wound healing is discussed.

Topics: 3',5'-Cyclic-AMP Phosphodiesterases; Animals; Collagen; Cyclic AMP; Cyclic GMP; Glycine; Male; Muscles; Protein Hydrolysates; Rats; Wound Healing; Wounds and Injuries

Changes in the content of cyclic nucleotides (cAMP and cGMP) in the wound tissues (muscles and granulations) were studied in experiments on rats. A wound with skin defect in the dorsal area with a crushed underlying muscle served as an experimental model. It was shown that the cAMP content in the muscular tissue rises twice: during the 1st day and to a greater measure on the 7th day. The cGMP content slightly increases on the 1-4th day, drops on the 7th day and increases cAMP concentration changes similarly to that inthe muscular tissue: it increases on the 7th day and drops on the 14th day. On the contrary, the cGMP content curve in granulations is more monotonous, only a slight increase being observed on the 7th day.

Topics: Animals; Cyclic AMP; Cyclic GMP; Male; Nucleotides, Cyclic; Rats; Time Factors; Wound Healing; Wounds and Injuries