cyclic-gmp and Splenic-Diseases

In the present work, we investigated the effects of aqueous extract of Maca (AEM) on energy metabolism and immunoregulation in spleen-deficient mice.. We established a cyclophosphamide-induced spleen-deficiency model with ginseng, a herb that strengthens splenic function, as a control. Sixty male Kunming mice were randomly divided among 5 groups: normal, model, ginseng control (1.5 g/kg), AEM high dose (1.5 g/kg), and AEM low dose (0.75 g/kg). All animals, except those in the normal group, were injected with cyclophosphamide to induce spleen deficiency. Furthermore, we investigated differences in the thermotropic behaviors of mice using the Animal Thermotropism Behavior Surveillance System to detect energy metabolism-related assays and immune regulation assays.. Mice given AEM exhibited tropism in response to hot plate exposure. AEM inhibited loss of body weight and immune organ atrophy caused by cyclophosphamide, increased the cAMP/cGMP ratio in blood, and enhanced the activities of Na. Maca possesses the Traditional Chinese Medicine (TCM) property of warm and appears to strengthen spleen function.

Topics: Animals; Cyclic AMP; Cyclic GMP; Cyclophosphamide; Cytokines; Energy Metabolism; Immunosuppressive Agents; Lepidium; Male; Mice; Plant Extracts; Splenic Diseases

In order to investigate the nature of Spleen deficiency and the mechanism of immunodepression due to Spleen deficiency and explore the pharmacological action of Chinese drugs of Yiqi Jianpi decoction (YQJP), the authors had established the rats model by administration Rhubarb. The preliminary results demonstrated that the symptoms manifested in rats were similar to those of Spleen deficiency syndrome. The changes of cyclic nucleotides in the spleen and plasma were unanimous. The cAMP level and the ratio of cAMP/cGMP decreased significantly while cGMP level increased significantly. Adenylate cyclase (AC) activity in the spleen reduced remarkably while cAMP-PDE activity had little changes. After the administration of YQJP, the symptoms of Spleen deficiency improved to normal extent. YQJP elevated the cAMP level, the ratio of cAMP/cGMP and AC activity while it lowered the cGMP level. The results showed that the changes of cyclic nucleotides level and the ratio of cAMP/cGMP were important targets of Spleen deficiency and that the action of YQJP followed the change of the ratio of cAMP/cGMP. The results of this study indicated that immunodepression of Rhubarb was due to depressing AC activity and reducing the ratio of cAMP/cGMP. The readjusting action of YQJP was concerned with AC system. This study supplied Spleen deficiency and YQJP with certain data in biochemical mechanism and pharmacological function.

Topics: Adenylyl Cyclases; Animals; Cyclic AMP; Cyclic GMP; Drugs, Chinese Herbal; Male; Medicine, Chinese Traditional; Phytotherapy; Plants, Medicinal; Rats; Rats, Inbred Strains; Rheum; Spleen; Splenic Diseases

Topics: Adult; Aged; Cyclic AMP; Cyclic GMP; Female; Gastric Mucosa; Humans; Male; Medicine, Chinese Traditional; Metaplasia; Middle Aged; Splenic Diseases; Stomach Neoplasms; Stomach Ulcer

Topics: Adult; Aged; Chronic Disease; Cyclic AMP; Cyclic GMP; Female; Gastric Mucosa; Humans; Male; Medicine, Chinese Traditional; Medicine, East Asian Traditional; Middle Aged; Splenic Diseases; Stomach Diseases

Topics: Adult; Aged; Cyclic AMP; Cyclic GMP; Female; Humans; Lymphocyte Activation; Male; Medicine, Chinese Traditional; Medicine, East Asian Traditional; Middle Aged; Splenic Diseases; Stomach Diseases