cyclic-gmp and Sarcoma-180

To confirm the effect of nutritional supportive therapy on cancer patients, 52 postoperative gastric cancer patients were selected and given 236 ED as nutritional therapy. Body weight and nutritional index before and after operation, complication, duration in hospital were compared in the experimental group and control. The results showed that there was a significant difference between the two groups. When inosine was given with 236 ED to 30 mice bearing S 180 sarcoma, tumor weight in the treated mice was less than that of the control; level of amino-acids which can stimulate tumor growth decreased whereas c-AMP content in the tumor tissue increased. Based on the above results, a rational nutritional support protocol for tumor patients and a pathomorphological classification of tumor in high, moderate and low nutritional status are proposed.

Topics: Amino Acids; Animals; Cyclic AMP; Cyclic GMP; Female; Food, Formulated; Humans; Inosine; Male; Mice; Postoperative Period; Prospective Studies; Sarcoma 180; Stomach Neoplasms

Intravenous injection of antigenic dose of sheep erythrocytes (SRBC) into mice caused a 150-200% increase in plasma cyclic GMP level within 5 min which continued for 60 min thereafter. Immunization by soluble antigens such as dextran sulfate and bovine serum albumin also elevated cyclic GMP level in plasma. The plasma cyclic GMP increased by antigen stimulation might be derived from immunocompetent cells. This assumption was supported by several lines of evidence as follows: (1) rat erythrocytes which are less antigenic for mice caused a relatively low response in terms of increase in cyclic GMP compared with SRBC, (2) this cyclic GMP response was abrogated in animals under immunosuppressive states where X-irradiated, azathioprine-treated or tumor-bearing mice were used. Mice pretreated with agents which block autonomic nervous system functions develop normal plasma cyclic GMP responses upon SRBC injection. Our observation in this report and many in vitro studies by other investigators suggested that plasma cyclic GMP elevated by antigen stimulation may be mainly derived from lymphoid cells as a consequence of the triggering of immune response.

Topics: Animals; Antigens; Autonomic Agents; Azathioprine; Cyclic GMP; Erythrocytes; Immunosuppressive Agents; Male; Mice; Rats; Rats, Inbred Strains; Sarcoma 180; Sheep; Time Factors

Topics: Animals; Antineoplastic Agents; Antiviral Agents; Arabinonucleotides; Arabinose; Carcinoma; Cell Line; Culture Techniques; Cyclic AMP; Cyclic GMP; HeLa Cells; Herpesviridae; Humans; Laryngeal Neoplasms; Mouth Neoplasms; Phosphoric Diester Hydrolases; Protein Kinases; Sarcoma 180