cyclic-gmp and Bronchial-Spasm

Sinesterases that intervene in cAMP and oGMP equilibrium in the cytoplasm of mast cells are activated by the adrenergic system through beta-receptors. A study was made on 96 atopic patients with mono sensitization to Dermatophagoides pteronissinus, in 3 groups. Radioligands were used to quantitate the number of beta receptors. In the control group mean no/cell was 541, bronchial symptomatic patients had 327 and nasal symptomatics 348. Asymptomatic patients were similar to controls. The results were similar with pollen-sensitive patients. Beta receptor decrease is a consequence of clinical allergy in atopic patients and is secondary to the immunological events that follow antigen-antibody reactions and cell membrane changes.

Topics: Adenylyl Cyclases; Allergens; Animals; Asthma; Bronchial Provocation Tests; Bronchial Spasm; Cyclic AMP; Cyclic GMP; Enzyme Activation; Humans; Hypersensitivity, Immediate; Mast Cells; Mites; Phosphoric Diester Hydrolases; Receptors, Adrenergic, beta; Rhinitis, Allergic, Seasonal; Signal Transduction

Topics: Adrenergic alpha-Antagonists; Asthma; Bronchial Spasm; Cyclic AMP; Cyclic GMP; Humans; Receptors, Adrenergic; Respiratory Function Tests

Topics: 3',5'-Cyclic-AMP Phosphodiesterases; Aminophylline; Asthma; Bronchial Spasm; Child; Cyclic AMP; Cyclic GMP; Drug Evaluation; Histamine; Humans; Prostaglandins E

The contents of histamine and cyclic nucleotides were studied in leukocytes before and after a bronchoprovocation test on patients with extrinsic asthma. The bronchospastic response correlated significantly with the reduction of the amount of histamine in leukocytes isolated after the provocation. The leukocytes cyclic AMP and cyclic GMP contents were slightly reduced after the provocation, while the plasma level of cyclic AMP was markedly increased. The beta-adrenoceptor response was tested on leukocytes, before the provocation. A normal elevation of the cyclic AMP content was obtained when the leukocytes were stimulated with isoprenaline, but after the provocation, isoprenaline had no significant effect. Only when a phosphodiesterase inhibitor was present did isoprenaline increase the cyclic AMP level in the postprovocation tests. It is suggested that the mediator release from the immunologic target cells is of importance for the bronchospastic response and that a desensitization of the beta-adrenoceptors of these cells is evident after bronchoprovocation.

Topics: 1-Methyl-3-isobutylxanthine; Adolescent; Adult; Allergens; Asthma; Bronchial Spasm; Cyclic AMP; Cyclic GMP; Female; Histamine; Histamine Release; Humans; Isoproterenol; Leukocytes; Male; Middle Aged; Peak Expiratory Flow Rate; Phosphodiesterase Inhibitors; Receptors, Adrenergic, beta

Topics: Animals; Bronchi; Bronchial Spasm; Cromolyn Sodium; Cyclic AMP; Cyclic GMP; Guinea Pigs; Humans; Lung

Topics: Adrenergic beta-Agonists; Adult; Bronchi; Bronchial Spasm; Bronchodilator Agents; Cyclic AMP; Cyclic GMP; Ephedrine; Epinephrine; Histamine Release; Humans; Isoproterenol; Mast Cells; Receptors, Adrenergic, alpha; Receptors, Adrenergic, beta; Respiratory Hypersensitivity; SRS-A; Sympathomimetics