cyanine-dye-3 and Brain-Injuries--Traumatic

Neural stem cells (NSCs) have been attractive donor sources for cell therapy in traumatic brain injuries (TBI). Monitoring the fate of transplanted cells, including the survival and differentiation, will provide vital information to assess the outcome during the therapy time course. However, the current labeling methods are based on the principles of cell endocytosis, demanding relatively high fluorescent probes concentration and long incubation time, which may affect the proliferation and differentiation of transplanted cells. In our study, an efficient and relatively fast labeling strategy for NSCs with Cy3 based on DNA hybridization was proposed for monitoring the fate of transplanted cells. The oligo[dA]

Topics: Animals; Brain Injuries, Traumatic; Carbocyanines; Cell Differentiation; Cell Survival; Disease Models, Animal; DNA; Mice; Neural Stem Cells; Nucleic Acid Hybridization; Stem Cell Transplantation