cyanine-dye-1 and Breast-Neoplasms

This report presents the synthesis and folate receptor target-specificity of amino-functionalized polyacrylamide nanoparticles (AFPAA NPs) for near-infrared (NIR) fluorescence imaging of cancer. For the synthesis of desired nano-constructs, the AFPAA NPs (hereafter referred to as NPs) were reacted with a NIR cyanine dye (CD) bearing carboxylic acid functionality by following our previously reported approach, and the resulting conjugate (NP-CD) on further reaction with folic acid (FA) resulted in a new nano-construct, FA-NP-CD, which demonstrated significantly higher uptake in folate receptor-positive breast cancer cells (KB+) and in folate receptor over-expressed tumors in vivo. The target-specificity of these nanoparticles was further confirmed by inhibition assay in folate receptor-positive (KB+) and -negative (HT-1080) cell lines. To show the advantages of polyacrylamide (PAA)-based NPs in folate receptor target-specificity, the CD used in preparing the FA-NP-CD construct was also reacted with folic acid alone and the synthetic conjugate (CD-FA) was also investigated for its target-specificity. Interestingly, in contrast to NPs (FA-NP-CD), the CD-FA conjugate did not show any significant in vitro or in vivo specificity toward folate receptors, showing the advantages of PAA-based nanotechnology in delivering the desired agent to tumor cells.

Topics: Acrylic Resins; Animals; Breast Neoplasms; Carbocyanines; Cell Line, Tumor; Female; Fibroblasts; Fibrosarcoma; Fluorescent Dyes; Folate Receptors, GPI-Anchored; Folic Acid; Glycoconjugates; Heterografts; Humans; Infrared Rays; KB Cells; Mice; Mice, Nude; Nanoparticles; Optical Imaging

Demarking lymph node networks is important for cancer staging in clinical practice. Here, we demonstrate in vivo dual-color photoacoustic lymphangiography using all-organic nanoformulated naphthalocyanines (referred to as nanonaps). Nanonap frozen micelles were self-assembled from two different naphthalocyanine dyes with near-infrared absorption at 707 nm or 860 nm. These allowed for noninvasive, nonionizing, high resolution photoacoustic identification of separate lymphatic drainage systems in vivo. With both types of nanonaps, rat lymph nodes buried deeply below an exogenously-placed 10 mm thick layer of chicken breast were clearly visualized in vivo. These results show the potential of multispectral photoacoustic imaging with nanonaps for detailed mapping of lymphatic drainage systems.

Topics: Acoustics; Animals; Breast Neoplasms; Carbocyanines; Cell Movement; Chickens; Color; Female; Humans; Lymph Nodes; Mice; Mice, Inbred BALB C; Micelles; Nanoparticles; Nanotechnology; Neoplasm Metastasis; Neoplasm Staging; Optics and Photonics; Rats; Rats, Sprague-Dawley; Sentinel Lymph Node Biopsy; Spectrophotometry