curcumin has been researched along with Staphylococcal-Infections* in 24 studies
1 review(s) available for curcumin and Staphylococcal-Infections
1 trial(s) available for curcumin and Staphylococcal-Infections
23 other study(ies) available for curcumin and Staphylococcal-Infections
| Article | Year |
|---|---|
Antibiofilm Effect of Curcumin Against Staphylococcus aureus Surface Wound Biofilm-Associated Infection: In Vitro and In Silico.
Biofilm is the consortia of the sessile group of microbial species that are adhered to the biotic and abiotic surfaces with the help of extracellular polymeric substances (EPS) and glycocalyx. A wound is a lesion on the epidermal surface that exposes the underlying tissues to the external environment and thus forms a region of proliferation for several species of Staphylococcus aureus. S. aureus is the most commonly observed nosocomial biofilm-forming organism that is responsible for the development of wound-associated infections. The biofilm prevents the penetration of the drug molecules thereby resulting in the development of antibiotic and multi-drug resistance among the organism. Thus, the use of alternative therapeutics has paved the path in the treatment of biofilm-associated infections. Curcumin has been used for the purpose of treating various illnesses from time immemorial. In this study, we observed that curcumin was able to bring about a reduction in the biofilm formed by S. aureus in the wound infection among the patients. The in silico studies revealed that curcumin possessed the ability to bring about interaction with the biofilm-forming proteins of S. aureus effectively. Topics: Anti-Bacterial Agents; Biofilms; Curcumin; Humans; Microbial Sensitivity Tests; Staphylococcal Infections; Staphylococcus aureus | 2023 |
Microfluidics Based Generation of Curcumin Loaded Microfibrous Mat against
The rapid increase in multidrug resistant biofilm infections is a major concern for global health. A highly effective therapy is required for the treatment of biofilm related infections. In this study, curcumin loaded alginate microfibers were generated by using the microfluidic technique. In this strategy, alginate microfibers are used as a carrier for the encapsulation of curcumin and then are irradiated with blue light to assess the efficacy of a combined therapy (blue light + curcumin) against drug resistant Topics: Biofilms; Curcumin; Humans; Methicillin-Resistant Staphylococcus aureus; Microfluidics; Photochemotherapy; Photosensitizing Agents; Staphylococcal Infections; Staphylococcus aureus | 2023 |
Natural versus synthetic curcuminoids as photosensitizers: Photobleaching and antimicrobial photodynamic therapy evaluation.
Antimicrobial photodynamic therapy (aPDT) has been studied as an alternative to combat bacterial resistance to the commonly used antibiotics. aPDT requires the use of a photosensitizer and curcumin is one of the more promising, though the usage of natural curcumin can be inconsistent in certain biomedical uses due to differences in soil condition and turmeric age, besides a large quantity of the plant is necessary to obtain useful amounts of the actual molecule. As such, a synthetic analogue is preferred as it is pure, and its components are better characterized. The present work studied photophysical differences in both natural and synthetic curcumin using photobleaching experiments and searched for whether differences existed in aPDT studies against Staphylococcus aureus. The results showed a faster O Topics: Anti-Bacterial Agents; Anti-Infective Agents; Curcumin; Diarylheptanoids; Humans; Photobleaching; Photochemotherapy; Photosensitizing Agents; Reproducibility of Results; Staphylococcal Infections; Staphylococcus aureus | 2023 |
Sonodynamic therapy exciting the herbal nanocomposite with spider-web-like effect to combat otitis media.
Otitis media, mainly caused by bacteria, is prevalent in young children and can cause hearing loss and growth retardation. Antibiotics are the most widely utilized treatment for otitis media, however, they can cause drug resistance and harmful side effects. In this study, we reported an antibacterial nanocomposite in combination with sonodynamic therapy that consists of herbal antibacterial agents such as Curcumin (CUR) and Tanshinone IIA (TSIIA), as well as Chitosan (CS), for the treatment of acute otitis media. CUR/TSIIA/CS nanocomposite (NC) with ultrasonic irradiation demonstrated that it could eliminate Staphylococcus aureus. In vivo experiments revealed that NC-mediated sonodynamic therapy had excellent antibacterial and anti-inflammatory activity, displaying a consistent performance comparable to ofloxacin. The therapeutic efficiency was attributed to capturing bacteria through spider-web-like effect and destroying bacteria through the reactive oxygen species generated under ultrasonic irradiation. Significantly, NC did not induce bacterial resistance and showed good biocompatibility. This study provides a novel strategy to develop an ultrasound-assisted nanocomposite with an enhanced antibacterial effect. Further, it unlocks new doors for the substitute of antibiotics to combat otitis media by establishing efficient therapeutic systems. Topics: Animals; Anti-Bacterial Agents; Bacteria; Child, Preschool; Chitosan; Curcumin; Humans; Nanocomposites; Otitis Media; Spiders; Staphylococcal Infections | 2022 |
Polyethylenimine-grafted mesoporous silica nanocarriers markedly enhance the bactericidal effect of curcumin against Staphylococcus aureus biofilm.
The recalcitrant nature of biofilms makes biofilm-associated infections difficult to treat in modern medicine. Biofilms have a high vulnerability to antibiotics and a limited repertoire of antibiotics could act on matured biofilms. This issue has resulted in a gradual paradigm shift in drug discovery and therapy, with anti-biofilm compounds being sought alongside new drug carriers. A potential solution to biofilm-associated infections is to employ antibiofilm treatments, which can attack biofilms from many fronts. Nanocarriers are promising in this regard because they can be entrapped within biofilm matrix, target biofilm matrix, and provide local drug delivery to inhibit biofilm formation. In this study, curcumin as an herbal extract was loaded onto hyperbranched polyethylenimine-grafted mesoporous silica nanoparticles (F-MSN-PEI/Cur) and antibiofilm investigations were performed. The F-MSN-PEI/Cur design has the potential to repurpose curcumin as an antibiofilm agent by increasing its solubility and lowering the required doses for the destruction of matured biofilms as well as suppressing biofilm development. Using imaging and spectroscopic techniques, we assessed the interaction of F-MSN-PEI/Cur with Staphylococcus aureus bacterial cells and determined the impact of F-MSN-PEI/Cur on eradicating matured biofilms and suppressing biofilm development. The F-MSN-PEI/Cur design is highly cytocompatible, as observed by the cytotoxicity screening investigations on L929 mouse fibroblast cell line. Our findings show that F-MSN-PEI/Cur design reduces the bacterial cell viability, inhibits biofilm formation, and induces biofilm eradication, which is attributed to F-MSN-PEI/Cur design having the potential to repurpose the antibiofilm activity of curcumin-herbal extract. Topics: Animals; Anti-Bacterial Agents; Biofilms; Curcumin; Drug Carriers; Mice; Microbial Sensitivity Tests; Polyethyleneimine; Silicon Dioxide; Staphylococcal Infections; Staphylococcus aureus | 2022 |
Antimicrobial photodynamic therapy (aPDT) with curcumin controls intradermal infection by Staphylococcus aureus in mice with type 1 diabetes mellitus: a pilot study.
Methicillin-resistant Staphylococcus aureus (MRSA) is one of the main pathogens that cause infections in diabetic individuals. In this paper, we report the outcomes of our investigation on the intradermal application of antimicrobial photodynamic therapy (PDT) with curcumin in an infection induced by MRSA ATCC 43300 strain in the ear of mice with Type 1 Diabetes Mellitus (T1DM). A solution containing 100 μg of curcumin was photoactivated ex vivo with a LED light (450 nm) delivering a fluency of 13.5 J/cm Topics: Animals; Biomarkers; Curcumin; Diabetes Mellitus, Type 1; Inflammation Mediators; Methicillin-Resistant Staphylococcus aureus; Mice; Mice, Inbred C57BL; Photochemotherapy; Pilot Projects; Skin Diseases, Bacterial; Staphylococcal Infections; Streptozocin | 2021 |
Curcumin-Chitosan Nanocomposite Formulation Containing
Because of its diverse range of use in several ethics of diagnosis and care of multiple diseases, nanotechnology has seen remarkable growth and has become a key component of medical sciences. In recent years, there has been rapid advancement in medicine and biomaterials. Nanomedicine aids in illness prevention, diagnosis, monitoring, and treatment.. The purpose of this work is to evaluate the antibacterial, anti-inflammatory, and cytotoxic capabilities of green produced silver nanoparticle with the addition of curcumin-assisted chitosan nanocomposite (SCCN) against wound pathogenic as reducing agents.. The plant extract of. The produced silver nanoparticle with the addition of curcumin-assisted chitosan nanocomposite (SCCN) has significant antibacterial activities against. This advancement in the field of biomaterials, which means nanocomposite, not only helps to reduce the harmful effects of pathogenic organisms while representing an environmentally benign material but it also shows to be a material with zero danger to humans and the environment. Topics: Anti-Bacterial Agents; Anti-Inflammatory Agents; Biocompatible Materials; Cell Line, Tumor; Chitosan; Curcumin; Humans; Metal Nanoparticles; Millettia; Nanocomposites; Nanotechnology; Particle Size; Plant Extracts; Pseudomonas aeruginosa; Pseudomonas Infections; Silver; Staphylococcal Infections; Staphylococcus aureus; Wound Healing | 2021 |
Combinatorial liposomes of berberine and curcumin inhibit biofilm formation and intracellular methicillin resistant
The increase in drug-resistant strains of Staphylococcus aureus, especially methicillin-resistant S. aureus (MRSA), has led to an increased rate of infection-related mortality. The emergence of drug resistance has rendered many antibiotics ineffective. The poor penetration and retention of antibiotics in mammalian cells lead to recurrent latent infections. Thus, there is an increasing need for biodegradable, non-toxic anti-infectives that are effective in treating MRSA infections. Phytochemicals such as berberine (BBR) and curcumin (CCR) have long been explored for their antibacterial activities, but their efficacy is often limited due to low bioavailability, water solubility, and poor cell penetration. When used in combination these antimicrobials did not show any synergistic effect against MRSA. Here, both of them were co-encapsulated in liposomes (BCL) and evaluated for biocompatibility, synergistic antimicrobial activity, intracellular infections, associated inflammation, and on biofilms formed by MRSA. Co-encapsulation of BBR and CCR in liposomes decreased their MICs by 87% and 96%, respectively, as compared to their free forms with a FICI of 0.13, indicating synergy between them. BCL inhibited the growth of MRSA and prevented biofilm formation better than free drugs. Co-culture studies showed that intracellular infection was reduced to 77% post BCL treatment. It also reduced the production of pro-inflammatory cytokines by macrophages following infection. The liposomes were found to be five times more efficient than clindamycin and can be used as a potential antimicrobial carrier against intracellular infections. Topics: Animals; Anti-Bacterial Agents; Berberine; Biofilms; Cells, Cultured; Curcumin; Humans; Inflammation; Liposomes; Methicillin-Resistant Staphylococcus aureus; Mice; Microbial Sensitivity Tests; Particle Size; Staphylococcal Infections; Surface Properties; THP-1 Cells | 2021 |
Sortase A-Inhibitory Coumarins from the Folk Medicinal Plant
Thirteen coumarins ( Topics: Aminoacyltransferases; Bacterial Proteins; Coumarins; Cysteine Endopeptidases; Fibrinogen; Fibronectins; Gram-Positive Bacteria; Membrane Proteins; Molecular Structure; Plants, Medicinal; Poncirus; Staphylococcal Infections; Staphylococcus aureus | 2020 |
Liposomes augment biological benefits of curcumin for multitargeted skin therapy.
Curcumin, a multi-targeting pharmacologically active compound, is a promising molecule for the treatment of skin inflammation and infection in chronic wounds. However, its hydrophobic nature remains to be a challenge in development of its pharmaceutical products, including dermatopharmaceuticals. Here we propose deformable liposomes (DLs) as a mean to overcome the curcumin limitations in skin treatment. We explored the properties and biological effects of curcumin containing DLs (curcumin-DLs) with varying surface charge by preparing the neutral (NDLs), cationic (CDLs) and anionic (ADLs) nanocarriers. The vesicles of mean diameter 200-300 nm incorporated high curcumin load mirroring the type of employed surfactant. Curcumin-CDLs provided the most sustained ex vivo penetration of curcumin through the full thickness human skin. Although the curcumin-CDLs were the most potent regarding the in vitro anti-inflammatory activity, all curcumin-DLs were superior to curcumin in solution (control). No cytotoxicity in human skin fibroblasts was detected. All DLs significantly inhibited bacterial Staphylococcus aureus and Streptococcus pyogenes growth in vitro. The curcumin-CDLs were found superior to other DLs. The incorporation of curcumin in DLs enabled both its sustained skin penetration and enhancement of its biological properties. Cationic nanocarriers enhanced the activities of curcumin to the greatest extent. Topics: Administration, Cutaneous; Cations; Cell Survival; Curcumin; Drug Carriers; Fibroblasts; Humans; Hydrophobic and Hydrophilic Interactions; Liposomes; Nanoparticles; Particle Size; Skin; Skin Absorption; Staphylococcal Infections; Staphylococcal Skin Infections; Staphylococcus aureus; Streptococcal Infections; Streptococcus pyogenes; Surface-Active Agents | 2019 |
Curcumin nanoparticles are a promising anti-bacterial and anti-inflammatory agent for treating periprosthetic joint infections.
Periprosthetic joint infections (PJIs) have a high incidence of recurrence after total joint replacement and are difficult to treat by debridement or antibiotic treatment. Curcumin is a natural product with anti-inflammatory and anti-bacterial properties. The low bioactivity of curcumin in water restricts its clinical application. Curcumin nanoparticles (CURN) were developed to overcome this limitation.. In this study, the therapeutic effects of CURN and their anti-inflammatory functions were investigated in a. CURN first attenuated the biofilm-induced expansion of myeloid-derived suppressor cells (MDSCs) and then regulated M1- and M2-phenotypic MDSC expression. Down-regulation of cytokines and reactive oxygen species was considered as the mechanism of CURN in reversing the suppression of T cell proliferation. The recovery of bone permeative destruction demonstrated that CURN enhanced therapeutic potency of vancomycin in vivo.. This is the first study to demonstrate that CURN may be useful for treating PJIs. Topics: Animals; Anti-Bacterial Agents; Anti-Inflammatory Agents; Biofilms; Cells, Cultured; Curcumin; Cytokines; Hip Joint; Male; Mice; Mice, Inbred C57BL; Nanoparticles; Prosthesis-Related Infections; Reactive Oxygen Species; Staphylococcal Infections; Staphylococcus aureus | 2019 |
Breathable hydrogel dressings containing natural antioxidants for management of skin disorders.
Traditional wound dressings are not effective enough to regulate the moisture content and remove excessive exudate from the environment. Wet wound dressings formed from hydrogels such as alginate are widely used in clinical practice for treatment of skin disorders. Here, we functionalize alginate dressings with natural antioxidants such as curcumin and t-resveratrol to render them both anti-inflammatory and antibacterial. The hydrogel maintains excellent mechanical properties and oxygen permeability over time. The release rate of the compounds from the hydrogels is assessed and their impact on bacterial and cellular growth is evaluated. The antioxidant compounds act as bactericidal agents and improve cell viability. The optimal concentration of active compounds in the engineered alginate-based dressings is determined. Topics: Alginates; Anti-Bacterial Agents; Anti-Inflammatory Agents, Non-Steroidal; Antioxidants; Bandages; Biocompatible Materials; Curcumin; Drug Delivery Systems; Drug Liberation; Humans; Hydrogels; Oxygen; Resveratrol; Skin Diseases; Staphylococcal Infections; Staphylococcus aureus | 2019 |
Flower-like curcumin-loaded folic acid-conjugated ZnO-MPA- βcyclodextrin nanostructures enhanced anticancer activity and cellular uptake of curcumin in breast cancer cells.
Non-spherical structures are beneficial to advance drug delivery effectiveness compared with common spherical ones, due to increased drug loading capability, improved bonding to a vascular wall, enhanced cellular uptake efficacy and prolonged circulation times. In this study, flower-like Zinc oxide-βcyclodextrin (βCD) nanostructures functionalized by 3-mercaptopropionic acid (MPA) as a non-spherical delivery system was successfully synthesized for aqueous delivery of curcumin (CUR) to enhance its targeting, bioavailability, and release profile. Terminal carboxyl functional groups were used for the conjugation of folic acid (FA) with the aim of active targeting to folate overexpressing breast cancer cells. The in vitro experimental study and mathematical modeling of CUR release revealed a sustained release with Fickian diffusion as the major release mechanism. MTT, colony formation and Annexin-V FITC/PI assays showed the superior anticancer effect of the system compared to free CUR against breast cancer cell line MDA-MB-231 by promoting the apoptotic respond with no cytotoxic effect on HEK293 normal cells. The efficacy of targeting strategy with FA moieties was demonstrated using the augmented cellular uptake of the FA-conjugated system on overexpressed folate receptor alpha (FRα) cells (MDA-MB-468 breast cancer cell line). Furthermore, loading of CUR to the delivery systems significantly lowered the MIC values (2.5 to 5-fold) against S. aureus and E. coli the infections of which are serious problems in cancer patients. According to the results of this study, the system can serve as a promising non-spherical delivery vehicle for enhancing bioavailability and targeting of hydrophobic anticancer agents in the future. Topics: 3-Mercaptopropionic Acid; Antineoplastic Agents; Apoptosis; beta-Cyclodextrins; Breast Neoplasms; Cell Line, Tumor; Curcumin; Drug Carriers; Escherichia coli; Escherichia coli Infections; Female; Folic Acid; HEK293 Cells; Humans; Nanostructures; Staphylococcal Infections; Staphylococcus aureus; Zinc Oxide | 2019 |
Nanocurcumin ameliorates Staphylococcus aureus-induced mastitis in mouse by suppressing NF‑κB signaling and inflammation.
Mastitis is the inflammation of the mammary glands caused by bacteria. It causes severe economic loss to dairy industry. Curcumin, a polyphenol obtained from turmeric, has considerable anti-inflammatory effect. Since it is rapidly eliminated from the body, its oral bioavailability is low. However, nanoformulation of curcumin significantly enhances its therapeutic efficiency by improving its oral bioavailability. We evaluated whether nanocurcumin could be more effective than normal curcumin against bovine Staphylococcus aureus mastitis in mouse model. Curcumin-loaded PLGA nanoparticles (CUR-NP) were prepared by solid-in-oil-in-water emulsion method. The mouse model of mastitis was induced by inoculation of a field strain of S. aureus (bovine mastitis isolate) on the 9th day of parturition through the duct of the mammary gland. CUR-NP and curcumin were given orally for 7 days (day 2 to day 8 of parturition) prior to S. aureus inoculation. We determined the levels of inflammatory cytokines and the mRNA expression of NF‑κB. S. aureus infection increased the levels of tumor necrosis factor‑α, interleukin‑1β and myeloperoxidase in mammary tissues and C-reactive protein in serum. Both CUR-NP and curcumin significantly attenuated the levels of these cytokines. However, comparatively, the ameliorative efficiency of CUR-NP was better than normal curcumin. S. aureus infection-induced NF‑κB mRNA expression was significantly reduced to the healthy control level by CUR-NP. Our study demonstrates that the nanoformulation of curcumin can reduce pro-inflammatory mediators in S. aureus-infected mammary tissues by improving NF‑κB signaling. Besides, compared to normal curcumin, this nanoformulation appears to be a better alternative against murine mastitis. Topics: Animals; C-Reactive Protein; Cattle; Curcumin; Disease Models, Animal; Female; Interleukin-1beta; Mammary Glands, Animal; Mastitis; Mice; Nanoparticles; NF-kappa B; Peroxidase; Polylactic Acid-Polyglycolic Acid Copolymer; Signal Transduction; Staphylococcal Infections; Staphylococcus aureus; Tumor Necrosis Factor-alpha | 2018 |
Bright carbon dots as fluorescence sensing agents for bacteria and curcumin.
Carbon dots (C-dots) are fluorescent nanomaterials that possess good photostability and low toxicity. They have been used as sensing probes and bioimaging agents for a variety of biological species. Numerous methods are available to generate C-dots. Nevertheless, simple and straightforward synthesis methods must be explored for the synthesis of C-dots from inexpensive, natural sources. In this study, we developed a simple method to generate C-dots from inexpensive chicken egg whites through a one-step heating reaction. The size of the generated C-dots was 3.3±0.4nm, and the quantum yield of the C-dots was as high as ∼43%. The as-prepared C-dots can be used as multicolor labeling agents for bacteria such as Staphylococcus aureus and Escherichia coli. Furthermore, the generated C-dots can be used as Förster resonance energy transfer sensing probes for curcumin, which is an active ingredient of turmeric and medicinal pigment. The feasibility of using the C-dots as selective sensing probes to determine the amount of curcumin from complex turmeric powder and condensed turmeric tablets is also demonstrated. Topics: Animals; Antineoplastic Agents; Biosensing Techniques; Carbon; Chickens; Curcuma; Curcumin; Escherichia coli; Escherichia coli Infections; Fluorescence Resonance Energy Transfer; Fluorescent Dyes; Quantum Dots; Staphylococcal Infections; Staphylococcus aureus; Tablets | 2017 |
Antibacterial activity of diacetylcurcumin against Staphylococcus aureus results in decreased biofilm and cellular adhesion.
Staphylococcus aureus infections have contributed to the global healthcare burden, particularly with regard to hospital-acquired meticillin-resistant S. aureus (MRSA) infections.. This study describes the antibacterial activity of diacetylcurcumin (DAC) against meticillin-susceptible S. aureus/MRSA biofilm formation, survival, metabolic activity and structure; its ability to prevent bacterial adhesion to human cells; and toxicity in Galleria mellonella larvae.. DAC showed excellent antibacterial activity, with MIC ranging between 17.3 and 34.6 µmol l-1, and minimum bactericidal concentration ranging between 69 and 277 µmol l-1. It significantly reduced bacterial biofilm survival - by 22-63 % (at MIC, 10×MIC or 100×MIC) as compared to the 25-42 % reduction by vancomycin (P<0.0001) - and severely affected biofilm cell structures, leading to damaged architecture and the formation of amorphous cell clusters. Treatment with DAC (MIC/4) decreased bacterial adhesion to HaCaT keratinocytes from 1 to 5 h (P<0.0001). Finally, DAC demonstrated low toxicity in G. mellonella at its effective anti-biofilm concentrations.. These findings open new avenues for the study of this curcumin derivative as an excellent prototype with anti-MRSA activity. Topics: Adhesins, Bacterial; Animals; Anti-Bacterial Agents; Biofilms; Curcumin; Humans; Keratinocytes; Larva; Methicillin-Resistant Staphylococcus aureus; Microbial Sensitivity Tests; Moths; Staphylococcal Infections; Staphylococcus aureus | 2017 |
Combination of Erythromycin and Curcumin Alleviates
Osteomyelitis is commonly caused by Topics: Animals; Anti-Bacterial Agents; Anti-Inflammatory Agents, Non-Steroidal; Curcumin; Disease Models, Animal; Drug Synergism; Drug Therapy, Combination; Erythromycin; Interleukin-6; Male; Methicillin-Resistant Staphylococcus aureus; Osteomyelitis; Rats; Rats, Wistar; Staphylococcal Infections; Tumor Necrosis Factor-alpha | 2017 |
Combination of Silver Nanoparticles and Curcumin Nanoparticles for Enhanced Anti-biofilm Activities.
Biofilm tolerance has become a serious clinical concern in the treatment of nosocomial pneumonia owing to the resistance to various antibiotics. There is an urgent need to develop alternative antimicrobial agents or combination drug therapies that are effective via different mechanisms. Silver nanoparticles (AgNPs) have been developed as an anti-biofilm agent for the treatment of infections associated with the use of mechanical ventilations, such as endotracheal intubation. Meanwhile curcumin, a phenolic plant extract, has displayed natural anti-biofilm properties through the inhibition of bacterial quorum sensing systems. The aim of this study was to investigate the possible synergistic/additive interactions of AgNPs and curcumin nanoparticles (Cur-NPs) against both Gram-negative (Pseudomonas aeruginosa) and Gram-positive (Staphylococcus aureus) microorganisms. The combination of AgNPs and Cur-NPs (termed Cur-SNPs) at 100 μg/mL disrupted 50% of established bacterial biofilms (formed on microtiter plates). However, further increase in the concentration of Cur-SNPs failed to effectively eliminate the biofilms. To achieve the same effect, at least 500 μg/mL Cur-NP alone was needed. Scanning electron microscopy (SEM) and confocal laser scanning microscopy (CLSM) revealed that combination therapy (Cur-SNPs) was the most potent to eradicate preformed biofilm compared to monodrug therapy. These agents are also nontoxic to healthy human bronchial epithelial cells (BEAS2B). Topics: Anti-Bacterial Agents; Anti-Infective Agents; Biofilms; Curcumin; Humans; Metal Nanoparticles; Microscopy, Confocal; Microscopy, Electron, Scanning; Models, Chemical; Pseudomonas aeruginosa; Silver; Staphylococcal Infections; Staphylococcus aureus | 2016 |
A comparative study on the antibacterial photodynamic efficiency of a curcumin derivative and a formulation on a porcine skin model.
The propagation of pathogens resistant to antibiotics around the globe has induced an urgent call for action: alternatives to conventional antibiotic therapy have to be developed to prevent a post-antibiotic catastrophe. This study focuses on the enhancement of Photodynamic Inactivation (PDI) of Gram(+) versus Gram(-) bacteria comparing a cationic derivative of curcumin (SACUR-3) to curcumin bound to polyvinylpyrrolidone (PVP-CUR) using an ex vivo porcine skin model to simulate an application on the human skin and foodstuff.. Porcine skin samples were inoculated with either Staphylococcus aureus or Escherichia coli and treated with either SACUR-3 or PVP-CUR at concentrations of 50 or 100 μM, respectively. Subsequent to blue light illumination (435 nm, 33.8 J cm(-2)) quantitative analyses were performed by counting the colony forming units. Furthermore, the localization of both photoactive compounds in the porcine skin was determined by fluorescence microscopy. PDI of S. aureus resulted in a reduction of 2.2 log10 steps if employing 50 μM of SACUR-3 and of 1.7 log10 steps with 50 μM of PVP-CUR. Phototoxicity towards E. coli was 3.3 log10 steps using 100 μM of SACUR-3 and 0.3 log10 steps for 100 μM of PVP-CUR. Both compounds do not exceed the stratum corneum of the skin.. A direct comparison of both approaches yields that the cationic curcumin derivative SACUR-3 is effective against Gram(+) and Gram(-) pathogens, whereas the formulation of PVP-CUR has a photokilling effect on the Gram(+) model strain only, but leaves the approval of curcumin as a food additive E100 unaffected. Our results suggest the applicability of SACUR-3-based PDI in dermatology, hand hygiene and food production. Topics: Animals; Anti-Bacterial Agents; Curcumin; Escherichia coli; Escherichia coli Infections; Humans; Photosensitizing Agents; Povidone; Skin; Staphylococcal Infections; Staphylococcus aureus; Swine | 2016 |
Sortase A Inhibitors: Recent Advances and Future Perspectives.
Here, we describe the most promising small synthetic organic compounds that act as potent Sortase A inhibitors and cater the potential to be developed as antivirulence drugs. Sortase A is a polypeptide of 206 amino acids, which catalyzes two sequential reactions: (i) thioesterification and (ii) transpeptidation. Sortase A is involved in the process of bacterial adhesion by anchoring LPXTG-containing proteins to lipid II. Sortase A inhibitors do not affect bacterial growth, but they restrain the virulence of pathogenic bacterial strains, thereby preventing infections caused by Staphylococcus aureus or other Gram-positive bacteria. The efficacy of the most promising inhibitors needs to be comprehensively evaluated in in vivo models of infection, in order to select compounds eligible for the treatment of bacterial infections in humans. Topics: Adamantane; Aminoacyltransferases; Animals; Anti-Bacterial Agents; Bacterial Adhesion; Bacterial Proteins; Benzoates; Carbolines; Cysteine Endopeptidases; Enzyme Inhibitors; Humans; Models, Molecular; Nitriles; Protein Conformation; Rhodanine; Small Molecule Libraries; Staphylococcal Infections; Staphylococcus aureus; Thiazoles; Thiones | 2015 |
Report-Isolation identification and control of vancomycin resistant Staphylococcus aureus.
Vancomycin resistant Staphylococcus aureus (VRSA) has been reported from many parts of the world including Asian countries. Hence, main objective of study was to evaluate the possible occurrence of VRSA in hospitals of Lahore city and to ensure the effectiveness of various substitute therapeutic options. A total of 150 samples of pus/wounds were collected from three hospitals of the city and VRSA were isolated and confirmed through recommended method of Clinical and Laboratory Standards Institute. Out of 51 (49.04%) methicillin resistant S. aureus (MRSA) isolates, 5 (9.8%) were found resistant to vancomycin. Minimum inhibitory concentration (MIC) of Linezolid (LZD), Moxifloxacin (MFX) and Clindamycin (CD) were calculated against VRSA isolates by broth microdilution test. All 5 (100%) isolates were susceptible to Linezolid and Clindamycin, while 4 (80%) were susceptible to Moxifloxacin. Ethanolic extracts of Turmeric, Mint, Coriander, Garlic, Kalonji, Cinnamon and Cloves illustrate average MIC values of 140.8 μg/mL, 563.2 μg/mL, 486.4 μg/mL, 614.4 μg/mL, 409.6 μg/mL, 281.6 μg/mL and 64 μg/mL, respectively against 5 VRSA strains. Concentration dependent increase in growth inhibition zones of ethanolic plant extract was recorded by agar well diffusion test. This study was helpful to find out the effective antibiotic against VRSA. Plant extracts encompass anti-staphylococcal activity and this finding demands necessity of further exploration of potential found in these natural herb. Topics: Acetamides; Anti-Bacterial Agents; Cinnamomum zeylanicum; Clindamycin; Curcuma; Fluoroquinolones; Garlic; Humans; Linezolid; Mentha; Methicillin-Resistant Staphylococcus aureus; Microbial Sensitivity Tests; Moxifloxacin; Nigella sativa; Oxazolidinones; Pakistan; Plant Extracts; Staphylococcal Infections; Staphylococcus aureus; Surgical Wound Infection; Syzygium; Vancomycin Resistance; Wound Infection | 2015 |
Upconversion nanoparticles conjugated with curcumin as a photosensitizer to inhibit methicillin-resistant Staphylococcus aureus in lung under near infrared light.
Curcumin has phototoxic effects on bacteria under <450 nm irradiation, but it is unstable in vivo and cannot exert effects on deep tissues. Near infrared light (NIR) is harmless to the body and has stronger penetration than visible light. In order to improve the effects of curcumin, upconversion nanoparticles conjugated with curcumin (UCNPs-curcumin) are designed to upconvert NIR to the excitation wavelength of curcumin. UCNPs-curcumin were synthesized using polyethyleneimine to combine curcumin and UCNPs, based on typical composition of lanthanide nitrates Re(NO)3 (Y:Yb:Er=78%:20%:2%) linked by ethylenediaminetetraacetic acid in sodium fluoride (NaF) matrix, to upconvert NIR to 432 nm light. The product was characterized by size distribution, thermogravimetric analysis, differential scanning calorimetry, and scanning electron microscopy. Growth inhibition of methicillin-resistant Staphylococcus aureus (MRSA) was not only measured in vitro but also investigated on MRSA-induced pneumonia in mice. The results showed that curcumin was covered by UCNPs to form stable nanoparticles whose average size was 179.5 nm and zeta potential was -33.7 mV in normal saline. The UCNPs-curcumin produced singlet oxygen, which reaches a stable level after 30 minutes of irradiation, and took effect on MRSA through bacterial cytoplasm leakage. They alleviated MRSA-induced pneumonia and reduced bacterial counts in lungs with 980 nm irradiation (0.5 W/cm(2)) on chests of mice. It is confirmed that the UCNPs-curcumin in lungs are activated under NIR irradiation and strengthen their antibacterial effects on MRSA. This research provides a new type of NIR photosensitizer, which plays an important role in phototoxic effects of curcumin in deep tissues under NIR. Topics: Animals; Colony Count, Microbial; Curcumin; Disease Models, Animal; Female; Infrared Rays; Lung; Male; Methicillin-Resistant Staphylococcus aureus; Mice; Nanoparticles; Oxygen; Photosensitizing Agents; Pneumonia, Bacterial; Staphylococcal Infections | 2014 |
Antimicrobial activity of curcumin-loaded myristic acid microemulsions against Staphylococcus epidermidis.
The bactericidal properties of myristic acid and curcumin were revealed in a number of studies. However, whether curcumin-loaded myristic acid microemulsions can be used to inhibit Staphylococcus epidermidis, which causes nosocomial infections, has not been reported. Our aim was to develop curcumin-loaded myristic acid microemulsions to inhibit S. epidermidis on the skin. The interfacial tension, size distribution, and viscosity data of the microemulsions were characterized to elucidate the physicochemical properties of the curcumin microemulsions. Curcumin distribution in neonate pig skin was visualized using confocal laser scanning microscopy. Dermal curcumin accumulation (326 µg/g skin) and transdermal curcumin penetration (87 µg/cm(2)/d) were obtained with the microemulsions developed herein. Curcumin at the concentration of 0.86 µg/mL in the myristic acid microemulsion could inhibit 50% of the bacterial growth, which was 12 times more effective than curcumin dissolved in dimethyl sulfoxide (DMSO). The cocktail combination of myristic acid and curcumin in the microemulsion carrier synergistically inhibited the growth of S. epidermidis. The results we obtained highlight the potential of using curcumin-loaded microemulsions as an alternative treatment for S. epidermidis-associated diseases and acne vulgaris. Topics: Administration, Topical; Animals; Anti-Bacterial Agents; Cross Infection; Curcumin; Emulsions; Humans; Myristic Acid; Skin; Skin Absorption; Staphylococcal Infections; Staphylococcus epidermidis; Swine | 2012 |