curcumin and Sarcoma-180

The current study aimed to investigate whether the novel folate (FT) modified nanoparticles (NPs) co-loaded with docetaxel (DT) and curcumin (CU) (named as FT-NPs) could enhance the delivery efficiency to tumor compared with the NPs without FT (non-targeted NPs).. FT-NPs were successfully formulated in this article. In vitro cytotoxic activity against A549 cells and in vivo antitumor activity of FT-NPs in S180 cell bearing mice were conducted. Cellular uptake test was used to evaluate uptake efficiency of FT-NPs. Histological observation was used to determine the lung security. Besides, the physical chemical properties such as stability, particle size, zeta potential, drug encapsulation efficiency, transmission electron microscopy (TEM) were also conducted.. FT-NPs exhibited stronger growth inhibition effects on A549 cells compared with non-targeted NPs, moreover, the novel FT-NPs indicated more effective antitumor efficacy in inhibiting tumor growth. Confocal laser scanning microscopy indicated that the uptake of FT-NPs was facilitated and effective. Histological observation meant that FT-NPs were biocompatible and appropriate for pulmonary administration.. These results confirmed that FT-NPs with relatively high drug loading capacity could effectively inhibit tumor growth and reduce toxicity. The novel FT-NPs could produce as an outstanding nanocarrier for the targeted treatment of cancers.

Topics: Animals; Antineoplastic Combined Chemotherapy Protocols; Cell Proliferation; Cell Survival; Chemistry, Pharmaceutical; Curcumin; Docetaxel; Drug Carriers; Folic Acid; HeLa Cells; Humans; Lung Neoplasms; Mice, Inbred C57BL; Nanomedicine; Nanoparticles; Polyesters; Polyethylene Glycols; Sarcoma 180; Solubility; Taxoids; Time Factors; Tumor Burden

To determine curcumin content by HPLC and observe the antitumor effects in vivo from different processed products of Curuma kwangsiensis.. We determined the content of curcumin by HPLC from different processed products of Curuma kwangsiensis and studied the anti-tumor effects in vivo by establishing S180 transplanted tumors model in mice.. The content of curcumin in different processed products of Curuma kwangsiensis as follows: primitive > vinegar-fried > vinegar-cooked. Different processed products had anti-tumor effects in the tumor-bearing mice. The vinegar-fried group and vinegar-cooked group showed obvious effect of inhibiting tumor growth than the primitive group.. Different processed methods may have some affection to the quality of Curuma kwangsiensis.

Topics: Animals; Antineoplastic Agents; Cell Line, Tumor; Chromatography, High Pressure Liquid; Curcuma; Curcumin; Female; Hot Temperature; Liver; Male; Mice; Neoplasm Transplantation; Rhizome; Sarcoma 180; Spleen; Technology, Pharmaceutical

The ability of the differentiation inducing agent sodium butyrate (NaB) alone or combined with plant-derived phenolic compounds to produce growth inhibition in human erythroleukemic cells was investigated. As a single agent, curcumin produced a marked inhibition of proliferation indicated by its low concentration used. The effect of phenolics on the cell cycle could probably contribute to the augmented antiproliferative activity of NaB. The present data show that quercetin produced synergistic effect in terms of cell killing in association with NaB. Both curcumin and ferulic acid potentiated NaB-induced reduction of cell number. When NaB was added before exposure to graded doses of quercetin it did induce a greater inhibitory effect. The combination of NaB and quercetin seems less effective on S180 ascites tumour cells. As a single agent quercetin was found to be the most efficacious on S180 tumour model.

Topics: Animals; Antineoplastic Combined Chemotherapy Protocols; Butyrates; Cell Division; Cell Survival; Coumaric Acids; Curcumin; Drug Synergism; Flow Cytometry; Humans; K562 Cells; Mice; Neoplasm Transplantation; Quercetin; Sarcoma 180