curcumin and Dermatomycoses

Dermatophytes are a group of fungal agents that can invade humans' keratinized tissues such as skin, nail, and hair, thereby causing dermatophyte infection (dermatophytosis) or ringworm. Some natural products have been reported to possess fungicidal effects. Hence, the present study investigated the effect of curcuminoids (CUR) and difluorinated curcumin (CDF) against clinical isolates of dermatophytes. CUR and CDF powders were evaluated against dermatophyte species including Trichophyton tonsurans (n = 21), T. mentagrophytes (n = 19), T. interdigitale (n = 18), Microsporum canis (n = 4), T. benhamiae (n = 1), and T. verrucosum (n = 1), based on the CLSI M38-A2 guideline. The minimum inhibitory concentration (MIC) ranges of CUR were 4-16, 8-16, 4-16, 8, 8, and 16 μg/ml for T. tonsurans, T. mentagrophytes, T. interdigitale, M. canis, T. benhamiae, and T. verrucosum, respectively. In addition, MIC ranges of CDF were obtained as 2-32, 4-16, 0.125-16, 8-16, 8, and 16 μg/ml, for T. tonsurans, T. mentagrophytes, T. interdigitale, M. canis, T. benhamiae, and T. verrucosum, respectively. CUR and CDF showed an inhibitory effect against dermatophyte isolates. CDF showed a stronger effect than CUR, especially against T. interdigitale. CUR and CDF have the capacity to be developed for use in dermatophytosis to augment existing preventative/therapeutic strategies.

Topics: Antifungal Agents; Arthrodermataceae; Curcumin; Dermatomycoses; Diarylheptanoids; Humans; Microsporum; Trichophyton

The frequent incidence of fungal infection and widespread of antibiotic resistance are emergent concerns in public health. Hence, there is a need to harness the potential of natural bioactive compounds from plant towards treatment of fungal infection. Combination effect of antibiotic creams with natural products from plants is prospective strategy to produce new antifungal agent. This study therefore, revealed antifungal effect of combined Antifungal Creams (AFCs) with Turmeric Essential Oil (TEO) or Aloe vera Gel (AVG).. Phytochemicals and bioactive compounds in TEO and AVG were revealed using GC-MS. Bioactive compounds in plant extracts were compared to known compounds in database library of National Institute of Standards and Technology (U.S.). Antifungal activity and synergistic effect of AFCs with TEO or AVG were carried out using agar well diffusion method.. Phenol, flavonoids, saponins, alkaloids, steroids, terpenoids and cardiac glycosides were present in TEO and AVG. GCMS revealed thirty-six (36) and eighteen (18) bioactive compounds in TEO and AVG, respectively. AFCs displayed zones of inhibition with values ranged from 5.0 to 14.3 mm, TEO was 5.0 to 11.0 mm and AVG was 8.0 to 11.7 mm against tested fungi. Minimum Inhibitory Concentration (MIC) by AFCs, TEO and AVG ranged from 1.25 to 10.0 mg/ml. Combinatory effects of AFCs with TEO or AVG revealed synergistic and indifferent properties.. Development of novel products using bioactive ingredients from plants with commercially available AFCs will serve as potential alternative therapy to cure dermatological infections with no side effects.

Topics: Antifungal Agents; Arthrodermataceae; Curcuma; Dermatomycoses; Drug Synergism; Drug Therapy, Combination; Gas Chromatography-Mass Spectrometry; Humans; Microbial Sensitivity Tests; Oils, Volatile; Plant Extracts; Plant Preparations

Topics: Animals; Antifungal Agents; Curcuma; Dermatomycoses; Fusarium; Hyphae; Mycelium; Plant Extracts; Rhizome; Trichechus manatus

Turmeric oil and curcumin, isolated from Curcuma longa L., were studied against fifteen isolates of dermatophytes, four isolates of pathogenic molds and six isolates of yeasts. The inhibitory activity of turmeric oil was tested in Trichophyton-induced dermatophytosis in guinea pigs. The results showed that all 15 isolates of dermatophytes could be inhibited by turmeric oil at dilutions of 1:40-1:320. None of the isolates of dermatophytes were inhibited by curcumin. The other four isolates of pathogenic fungi were inhibited by turmeric oil at dilutions of 1:40-1:80 but none were inhibited by curcumin. All six isolates of yeasts tested proved to be insensitive to both turmeric oil and curcumin. In the experimental animals, turmeric oil (dilution 1:80) was applied by dermal application on the 7th day following dermatophytosis induction with Trichophyton rubrum. An improvement in lesions was observed in 2-5 days and the lesions disappeared 6-7 days after the application of turmeric oil.

Topics: Animals; Antifungal Agents; Arthrodermataceae; Candida; Cell Division; Curcuma; Curcumin; Dermatomycoses; Female; Guinea Pigs; Male; Miconazole; Microbial Sensitivity Tests; Plant Extracts; Plant Oils; Plants, Medicinal; Thailand