cufe2o4 and Prostatic-Neoplasms

Our previously prepared ternary nanocomposite TNT/CuFe₂O₄/Zn-Fe was highly engulfed by PC-3 cells, activated cytotoxicity that was dosage and time-subordinated, and demonstrated morphological alteration, which is one of the common characteristics of apoptotic cells. This prolonged study aimed to investigate other items. The study performed assays as Annexin V-FITC, flow cytometry, DNA ladder electrophoresis, and ROS assay for apoptosis detection, cell cycle analysis, DNA fragmentation, and ROS generation, respectively. In the PC-3-treated cells, the early and late phases of apoptosis with different percentages and DNA fragmentation were determined. Besides, the PC-3 cell cycle revealed the three major cell distribution different phases of the cycle (G1, S, and G2/M), and the Sub G1, which corresponded to apoptotic cells. The results proved the presence of ROS that triggered the intrinsic apoptotic pathway, which was confirmed through a decrease in (Bcl-2), the release of cytochrome c, activation of caspase-9, and caspase-3. To conclude, the ternary nanocomposite TNT/CuFe₂O₄/Zn-Fe achieved biochemical features alterations and could induce intrinsic apoptosis of PC-3 cells. The planned work of the current research will illuminate the arrested phase in the cell cycle through studying tumor suppressor genes such as p53 and Retinoblastoma RB,

Topics: Apoptosis; Caspase 3; Cell Line, Tumor; Copper; Ferrous Compounds; Humans; Male; Nanocomposites; Prostatic Neoplasms; Proto-Oncogene Proteins c-bcl-2; Zinc