crustacean-cardioactive-peptide and Chagas-Disease

crustacean-cardioactive-peptide has been researched along with Chagas-Disease* in 2 studies

Other Studies

2 other study(ies) available for crustacean-cardioactive-peptide and Chagas-Disease

Article	Year
Isolation, cloning and expression of the Crustacean Cardioactive Peptide gene in the Chagas' disease vector, Rhodnius prolixus. Peptides, 2011, Volume: 32, Issue:3 The blood-gorging bug, Rhodnius prolixus, is a major vector of Chagas' disease in Central and South America. We have cloned and characterized the crustacean cardioactive peptide (CCAP) gene in R. prolixus. The RhoprCCAP gene contains five exons and four introns, and encodes a 129 amino acid prepropeptide, which following post-translation processing, produces CCAP. The predicted RhoprCCAP amino acid sequence is identical to CCAP of crustaceans and other insects, i.e. it is highly conserved. RhoprCCAP mRNA is observed in the central nervous system (CNS) using reverse transcriptase (RT) PCR, but not in the gut and salivary glands. In situ hybridization reveals that the expression of CCAP mRNA is localized to a small number of dorsally situated bilaterally paired neurons within the CNS. Topics: Animals; Base Sequence; Blotting, Northern; Central Nervous System; Chagas Disease; Disease Vectors; In Situ Hybridization; Molecular Sequence Data; Neuropeptides; Rhodnius; Sequence Alignment; Sequence Homology, Amino Acid	2011
Crustacean cardioactive peptide in the Chagas' disease vector, Rhodnius prolixus: presence, distribution and physiological effects. General and comparative endocrinology, 2011, Oct-01, Volume: 174, Issue:1 Crustacean cardioactive peptide (CCAP), a cyclic nonapeptide (PFCNAFTGCamide), has multifunctional roles in insects including stimulating visceral and cardiac muscle contraction, and regulating ecdysis. Previously, we have sequenced the cDNA for CCAP from Rhodnius prolixus central nervous system (CNS) and shown expression of the CCAP transcript in neurons of the CNS. In the present study, we have biochemically identified and sequenced CCAP from 5th instar R. prolixus CNS using matrix-assisted laser desorption ionization-time of flight-tandem mass spectrometry, and mapped CCAP-like immunoreactivity in the CNS and peripheral tissues of 5th instar R. prolixus. Physiologically, the hindgut of R. prolixus was found to be sensitive to CCAP, showing dose-dependent increases in contractions with threshold at 5 × 10(-9) M and maximum response at 10(-7) M CCAP. Also, CCAP was found to increase the frequency of the heartbeat in a reversible, dose-dependent manner, with threshold close to 10(-11) M and maximum response at 10(-10) M CCAP. Topics: Animals; Central Nervous System; Chagas Disease; Disease Vectors; Gastrointestinal Tract; Heart; Immunohistochemistry; Neurons; Neuropeptides; Rhodnius; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization	2011

Article

Year

Isolation, cloning and expression of the Crustacean Cardioactive Peptide gene in the Chagas' disease vector, Rhodnius prolixus.

Peptides, 2011, Volume: 32, Issue:3

The blood-gorging bug, Rhodnius prolixus, is a major vector of Chagas' disease in Central and South America. We have cloned and characterized the crustacean cardioactive peptide (CCAP) gene in R. prolixus. The RhoprCCAP gene contains five exons and four introns, and encodes a 129 amino acid prepropeptide, which following post-translation processing, produces CCAP. The predicted RhoprCCAP amino acid sequence is identical to CCAP of crustaceans and other insects, i.e. it is highly conserved. RhoprCCAP mRNA is observed in the central nervous system (CNS) using reverse transcriptase (RT) PCR, but not in the gut and salivary glands. In situ hybridization reveals that the expression of CCAP mRNA is localized to a small number of dorsally situated bilaterally paired neurons within the CNS.

Topics: Animals; Base Sequence; Blotting, Northern; Central Nervous System; Chagas Disease; Disease Vectors; In Situ Hybridization; Molecular Sequence Data; Neuropeptides; Rhodnius; Sequence Alignment; Sequence Homology, Amino Acid

2011

Crustacean cardioactive peptide in the Chagas' disease vector, Rhodnius prolixus: presence, distribution and physiological effects.

General and comparative endocrinology, 2011, Oct-01, Volume: 174, Issue:1

Crustacean cardioactive peptide (CCAP), a cyclic nonapeptide (PFCNAFTGCamide), has multifunctional roles in insects including stimulating visceral and cardiac muscle contraction, and regulating ecdysis. Previously, we have sequenced the cDNA for CCAP from Rhodnius prolixus central nervous system (CNS) and shown expression of the CCAP transcript in neurons of the CNS. In the present study, we have biochemically identified and sequenced CCAP from 5th instar R. prolixus CNS using matrix-assisted laser desorption ionization-time of flight-tandem mass spectrometry, and mapped CCAP-like immunoreactivity in the CNS and peripheral tissues of 5th instar R. prolixus. Physiologically, the hindgut of R. prolixus was found to be sensitive to CCAP, showing dose-dependent increases in contractions with threshold at 5 × 10(-9) M and maximum response at 10(-7) M CCAP. Also, CCAP was found to increase the frequency of the heartbeat in a reversible, dose-dependent manner, with threshold close to 10(-11) M and maximum response at 10(-10) M CCAP.

Topics: Animals; Central Nervous System; Chagas Disease; Disease Vectors; Gastrointestinal Tract; Heart; Immunohistochemistry; Neurons; Neuropeptides; Rhodnius; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization

2011