cp-544326 and Corneal-Diseases

To assess the effect of topical taprenepag isopropyl on each layer of the cornea by confocal microscopy.. Thirty-two ocular hypertensive or glaucoma patients were randomized into a 2-period, crossover study of 14 days of 0.1% taprenepag alone and in unfixed combination with 0.005% latanoprost (combination therapy). Baseline and sequential slit-lamp biomicroscopy, fluorescein staining, central ultrasonic pachymetry, and confocal microscopy were performed. Confocal images were analyzed for the density of the central superficial and basal epithelium, midstromal keratocytes, and endothelium, as well as endothelial coefficient of variation and percentage of hexagonal cells, and reflectivity of anterior stromal and midstromal layers.. Corneal staining increased from baseline, reaching a peak at day 13 (69% and 63% of subjects treated with monotherapy and combination therapy, respectively), which resolved by day 35. A statistically significant increase in mean corneal thickness for both eyes and both treatments occurred on days 7 and 13 (range, 20-27 μm; P < 0.001) but recovered (≤ 6 μm) by day 35. No statistically significant changes were observed in the basal epithelial, midstromal, or endothelial cells. Mean ratio of average reflectivity of anterior stroma to midstroma increased on days 13 and 35 in period 1 for each treatment (range, 1.2-1.9; P < 0.001), and this increase persisted during period 2.. Anterior stromal reflectivity may remain increased even when biomicroscopic and confocal images of corneal layers remain normal or have recovered after topical taprenepag. This subclinical measure may be useful to detect a persistent adverse effect of a topical agent on the cornea.

Topics: Acetates; Administration, Topical; Aged; Aged, 80 and over; Cell Count; Corneal Diseases; Corneal Keratocytes; Corneal Pachymetry; Corneal Stroma; Cross-Over Studies; Double-Blind Method; Drug Therapy, Combination; Endothelium, Corneal; Epithelium, Corneal; Glaucoma, Open-Angle; Humans; Latanoprost; Microscopy, Confocal; Middle Aged; Ocular Hypertension; Ophthalmic Solutions; Prostaglandins F, Synthetic; Receptors, Prostaglandin E, EP2 Subtype; Refraction, Ocular; Sulfonamides; Visual Acuity