cord-factors and Fibrosarcoma

Topics: Animals; Cord Factors; Fibrosarcoma; Glycolipids; Lung Neoplasms; Male; Mice; Sarcoma, Experimental; Skin Neoplasms

Topics: Animals; Cord Factors; Emulsions; Endotoxins; Fibrosarcoma; Glycolipids; Male; Mice; Mice, Inbred C3H; Mineral Oil; Neoplasm Transplantation; Neoplasms, Experimental; Turpentine

Intratumoral injection of ultrasonically prepared emulsions of the synthetic glycolipid methly 6-O-(2-tetradecyl-3-hydroxyoctadecanoyl)-alpha-D-glucopyranoside (designated C39) induced complete regression of transplants of a syngeneic murine fibrosarcoma in most of the treated animals as did 6,6'-di-O(2-tetradecyl-3-hydroxyoctadecanoyl)-alpha,alpha,-trehalose (designated C76) in a previous study. The C76 compound, about twice the molecular weight of C39, was more effective therapeutically than the smaller molecule. Ultrasonically prepared emulsions of C39 and C76 were not toxic when given intravenously. Intravenously administered emulsions of C39 prepared by mechanical grinding were more toxic, but less granulomagenic, than those containing C76. Squalane and squalene, but not peanut oil, were effective substitutes for mineral oil as carriers of C39 in the treatment of the tumor.

Topics: Animals; Chemical Phenomena; Chemistry; Cord Factors; Emulsions; Fibrosarcoma; Glycolipids; Granuloma; Male; Methylcholanthrene; Mice; Neoplasm Transplantation; Sarcoma, Experimental

Injection of emulsified 6,6'-di-O-2-tetradecyl-3-hydroxyoctadecanoyl-a, a trehalose designated C76, a synthetic analogue of the mycobacterial glycolipid trehalose-6,6'-dimycolate (TDM), into transplants of an established, syngeneic murine fibrosarcoma induced complete regression of tumor in a number of animals. The number of animals in which tumor regressed completely dependent on the amount of oil in the emulsion. On a weight basis, C76 was at least as active as TDM. Intralesional injection of an emulsified mixture of C76 and endotoxin (ET) or of TDM and ET caused regression of an established transplant of a guinea-pig hepatoma in syngeneic animals. In mice, intravenously administered emulsions of C76 were less toxic and less granulomagenic than those made with TDM.

Topics: Animals; Cell Line; Cord Factors; Disaccharides; Drug Evaluation, Preclinical; Emulsions; Fibrosarcoma; Glycolipids; Guinea Pigs; Injections; Injections, Intravenous; Male; Mice; Neoplasm Transplantation; Remission, Spontaneous; Sarcoma, Experimental; Transplantation, Isogeneic; Trehalose