concanavalin-a and Taeniasis

Peritoneal infection with Taenia crassiceps cysticerci of naturally resistant (C57BL/10J and C57BL/6J) and susceptible (BALB/cAnN) mice induces a cellular immune depression. T-cell proliferation in response to concanavalin A (ConA) or anti-CD3 was significantly depressed in infected mice of all strains tested. However, in resistant mice, the diminished response to ConA was transient and animals recovered normal responsiveness at day 40, whereas susceptible mice remained suppressed throughout the 40 days of the experiment. In contrast, the proliferative response to anti-CD3 was lower in infected mice than in noninfected controls regardless of differences in natural susceptibility of the strains. Intraperitoneal injection of mice with a parasite extract also induced a depression of the response to ConA, although not as strong as that produced by the parasite itself. This depression is not due to direct effects by parasite antigens over host lymphocytes, as proliferation is not affected by the presence of cysticercal antigens added in vitro. Diminished interleukin-2 production during the parasitosis accounts at least in part for the diminished responses to ConA. A primary infection favors parasite establishment after a second challenge, pointing to the relevance of the immunodepression in generating a host environment favorable to the parasite.

Topics: Animals; CD3 Complex; Concanavalin A; Immune Tolerance; Interleukin-2; Lymphocyte Activation; Mice; Mice, Inbred BALB C; Mice, Inbred C57BL; Recombinant Proteins; T-Lymphocytes; Taeniasis

This study was designed to test the accessory function of macrophages after activation with products of Taenia multiceps coenuri. Activation was carried out by intraperitoneal injection of mice with coenurus fluid or protoscolex culture supernatant, and function was assessed by adding these macrophages in progressively increasing numbers to macrophage-depleted lymphocyte cultures transforming under the influence of plant mitogens or coenurus-fluid mitogen. In contrast to normal macrophages, which have a progressively enhancing action on the above reactions, parasite-activated macrophages at similar concentrations were progressively inhibitory. However, low concentrations of the activated macrophages enhanced mitosis as well as, or better than, normal. Lymph node cells from injected mice showed abnormal response to macrophage-derived signals. In particular there was subnormal reaction to macrophages in the presence of coenurus mitogen. These results suggest that T. multiceps coenuri may survive in the host because of their ability to reduce effective interaction between lymphocytes and accessory cells.

Topics: Animals; Antigen-Presenting Cells; Concanavalin A; Lymphocyte Activation; Macrophage Activation; Macrophages; Mice; Mice, Inbred BALB C; Mitogens; Peritoneal Cavity; Phytohemagglutinins; T-Lymphocytes; Taenia; Taeniasis

The effect of taeniid infection on the in vitro cellular response of the host was investigated. Infections of Taenia taeniaeformis decreased the ability of spleen cells from susceptible C3H/He mice to respond to the T-cell mitogen concanavalin A (Con A) as early as 2 days postinfection (pi) reaching a suppression peak at day 12 pi. Similar experiments performed with spleen cells from infected BALB/c mice, resistant to the infection, revealed little or no suppression of Con A stimulation. The results suggested that susceptibility to the parasite may be due to its ability to induce a partial suppression of the host's immune system. The role of adherent splenocytes from infected C3H/He mice in the production of a deficient response to Con A during early infection was studied by coculturing experiments. These experiments demonstrated that adherent populations from infected mice did not play a direct role in the Con A-suppressor mechanisms. Concomitant with the suppressor activity an increased background proliferation was observed with nonstimulated splenocytes from C3H/He mice infected with T. taeniaeformis. Plasma from infected mice was able to suppress the response of normal spleen cells to Con A and to stimulate a proliferative response in cultured splenocytes from noninfected animals. The results suggest the presence of factors in the plasma of infected mice which may be modulating the immune response to the parasite.

Topics: Animals; Concanavalin A; Female; Immunity, Cellular; Larva; Lymphocyte Activation; Mice; Mice, Inbred BALB C; Mice, Inbred C3H; T-Lymphocytes; Taenia; Taeniasis

Splenocytes from rats infected with Taenia taeniaeformis showed an early decreased proliferative response to the mitogen concanavalin A with larval growth. When larvae culture supernatant (in vitro product) was added to normal rat splenocytes, there was a decrease in the proliferative response to concanavalin A and phytohemagglutinin. The ability of infected rat splenocytes to produce interleukin-2 was decreased with larval growth. Addition of in vitro product to culture medium significantly depressed interleukin-2 production by normal as well as infected rat spleen cells. Culturing normal rat splenocytes with in vitro product for 3 days induced a suppressor cell population. When these cultured cells were admixed with fresh normal rat splenocytes plus concanavalin A, the proliferative response of the fresh cells was significantly reduced. The present results suggest that in vitro product secreted by larvae in hepatic cysts causes subversion of the cellular immune response in the host. Induction of a suppressor cell population could suppress interleukin-2 production which may lead to the inhibition of differentiation and proliferation of specific cytotoxic T lymphocytes.

Topics: Animals; Calcimycin; Cells, Cultured; Concanavalin A; Female; Interleukin-1; Interleukin-2; Lymphocyte Activation; Mitogens; Rats; Rats, Inbred Strains; Spleen; T-Lymphocytes, Regulatory; Taenia; Taeniasis