concanavalin-a and Sarcoma

This study aimed to investigate the effects of a health product Squina (Diacylglyceryl Ether) (DAGE) on innate and adaptive immunity in mice. Both ex vivo/ in vivo mouse models and an in vitro system using cultured mouse splenocytes were recruited for the assessment of innate and adaptive immunity. For the innate immune response, DAGE extract treatment enhanced the LPS-induced IL1-β secretion by activated macrophages in vitro and long-term DAGE treatment (0.18 and 1.8 g/kg × 20 doses) elevated the phagocytic activity of macrophages, as well as natural killer cell activity in mice. The DAGE-induced increase in macrophage phagocytic and natural killer cell activities were accompanied by the suppression of tumor growth in Sarcoma-180 cell-inoculated mice. For the adaptive immune response, long-term DAGE treatment enhanced the splenocyte index and concanavalin A- stimulated proliferation ex vivo in mice. Consistently, the incubation with DAGE extract potentiated the concanavalin A-stimulated proliferation in mouse splenocytes in vitro. In conclusion, the results show that long-term DAGE treatment produces stimulatory effects on both innate and adaptive immunity in mice.

Topics: Adaptive Immunity; Animals; Antineoplastic Agents; Cell Proliferation; Cells, Cultured; Concanavalin A; Diglycerides; Dose-Response Relationship, Drug; Female; Fish Oils; Food, Organic; Immunity, Innate; Interleukin-1beta; Killer Cells, Natural; Lipopolysaccharides; Lymphocyte Activation; Macrophages; Male; Mice; Mice, Inbred ICR; Mitogens; Neoplasm Transplantation; Phagocytosis; Sarcoma; Spleen; Tumor Cells, Cultured

The responses to Concanavalin A (Con A) of peripheral blood mononuclear cells were studied in 22 patients with malignant bone and soft tissue sarcoma. The decreases of response were shown in many cases depending on their clinical stages. The responses were increased by removal of the adherent cells from mononuclear cells by passing through a Sephadex G-10 column in 7 of 13 cases tested. The enhancement of responses to Con A was also found when indomethacin, a inhibitor of prostaglandin biosynthesis, was added to mononuclear cell suspensions. However, no additional increase of the responses was found when indomethacin was supplemented to the adherent cell-depleted mononuclear cell suspensions. Since it has been previously shown that prostaglandin was primarily produced from macrophages, the author concluded that the suppressor cells affecting on the lymphocyte transformation probably belong to monocyte-macrophage series and their suppressive effect may be mediated by prostaglandin. In the normal control, the responses of mononuclear cells to Con A stimulation were rather reduced by removal of the adherent cells, suggesting that these suppressor cells were contained only in mononuclear cells of the tumor-bearing hosts.

Topics: Bone Neoplasms; Cell Adhesion; Concanavalin A; Humans; Indomethacin; Monocytes; Sarcoma; Soft Tissue Neoplasms; T-Lymphocytes, Regulatory

Forty-seven patients with mesenchymal sarcoma seen at the Mayo Clinic were tested for humoral and cellular immunologic responsiveness by means of a humoral cytotoxicity test, skin tests for delayed-type hypersensitivity, and lymphocyte transformation with concanavalin A, phytohemagglutinin, and pokeweed. Prospective follow-up of the clinical and laboratory results revealed that patients with low humoral cytotoxic indices, anergic skin test responses, or low lymphocyte transformation responsiveness tended to have the worst short-term clinical outlook.

Topics: Adolescent; Adult; Aged; Bibliographies as Topic; Child; Concanavalin A; Cytotoxicity Tests, Immunologic; Female; Humans; Hypersensitivity, Delayed; Immunity; Immunity, Cellular; Lectins; Lymphocyte Activation; Male; Middle Aged; Mitogens; Prognosis; Sarcoma; Skin Tests; Soft Tissue Neoplasms

Topics: Animals; Cell Line; Cell Transformation, Neoplastic; Cells, Cultured; Chromosomes; Colchicine; Concanavalin A; Fibroblasts; Floxuridine; Gammaretrovirus; Genetic Variation; Idoxuridine; Mice; Mice, Inbred Strains; Mutation; RNA-Directed DNA Polymerase; Sarcoma

Topics: Adult; Agglutination; Cell Line; Cell Transformation, Neoplastic; Cells, Cultured; Concanavalin A; Depression, Chemical; Fibroblasts; Glioma; Glucose; Glutaral; Humans; Hyaluronoglucosaminidase; In Vitro Techniques; Lactose; Lectins; Lung; Methods; Neuroglia; Plant Lectins; Plants, Toxic; Ricinus; Sarcoma; Stimulation, Chemical; Temperature; Trypsin

Topics: Adenocarcinoma; Concanavalin A; Humans; Hypersensitivity, Delayed; Immunologic Memory; Lectins; Lymphocyte Activation; Lymphocyte Culture Test, Mixed; Melanoma; Neoplasms; Nitrobenzenes; Sarcoma; Skin Tests

Non-virus-producing NIH/3T3 cells transformed by the murine sarcoma virus are agglutinated by conconavalin A to the same low level as normal NIH/3T3 cells. Infection with the murine leukemia virus greatly increases the agglutination of transformed cells but not that of normal cells. These data suggest that the morphological expression of cell transformation and the surface alterations associated with increased cell agglutination are controlled by the expressions of different sarcoma virus genes.

Topics: Agglutination; Animals; Cell Line; Cell Transformation, Neoplastic; Clone Cells; Concanavalin A; Fibroblasts; Gammaretrovirus; Genotype; Leukemia Virus, Murine; Mice; Mice, Inbred Strains; Sarcoma

A variety of epithelial cells and fibroblasts fail to move over one another's upper surfaces in culture, resulting in monolayering. The failure of seeded fibroblasts to adhere to and spread on epithelial cell surfaces suggests that monolayering in culture is due to the lack of adhesion of the upper cell surface, at least of epithelial cells. Seeded fibroblasts and postmitotic, rounded fibroblasts likewise fail to spread on the upper surfaces of spread fibroblasts, suggesting that the inability of the upper cell surface to support spreading may be a general phenomenon. Inert particles and cell processes do not adhere directly to the upper cell surface. However, they can initiate adhesions to the surface at a cell's free margin, suggesting a variation of adhesive properties over a cell's surface.

Topics: Animals; Carcinoma; Cell Adhesion; Cell Aggregation; Cell Division; Cell Line; Cell Membrane; Cell Movement; Concanavalin A; Cornea; Epithelial Cells; Epithelium; Erythrocytes; Fibroblasts; Gizzard, Non-avian; Humans; Latex; Microscopy, Phase-Contrast; Microspheres; Mitosis; Motion Pictures; Mouth Neoplasms; Sarcoma; Skin; Time Factors