concanavalin-a and Periodontal-Diseases

A complex of colloidal gold and concanavalin A (CG-Con A) with various biological properties and high ultrastructural resolution was applied into the sulcus of rat molar gingiva and traced with an electron microscope for three hours to examine the cytological changes occurring in the cells of the junctional epithelium (JE) during penetration of extrinsic irritants, and to determine the roles of JE cells in such a circumstance. While the penetration of CG-Con A was impeded on the surface of keratinized oral gingival/sulcular epithelium, CG-Con A penetrated swiftly through JE into the connective tissue. In the process of penetration, CG-Con A was taken up by lysosomal and vacuolar structures of JE cells in which degenerative changes were often provoked. Degeneration of JE cells was seen selectively in the second and/or third cell layers from the innermost cell layer of JE. It was assumed that JE cells by their phagocytic activity might participate in the first line of defense against extrinsic irritants. On the other hand, the phagocytic activity of JE cells seems also to be involved in tissue destruction, if the amount and/or toxicity of irritants exceed the dissimilating capacity of JE cells.

Topics: Animals; Concanavalin A; Epithelial Attachment; Gingiva; Gold; Immunohistochemistry; Male; Periodontal Diseases; Periodontium; Rats; Rats, Inbred Strains

Topics: Adolescent; Adult; Antigens; Concanavalin A; Dental Plaque; Female; Fetal Blood; Humans; Infant, Newborn; Lymphocyte Activation; Lymphocytes; Periodontal Diseases; Streptolysins

Dental plaque deposits are known to be potent stimulants of lymphocyte transformation in patients with periodontal disease but not in normal subjects. Since plaque deposits consist mainly of whole bacteria, the cell walls of the most commonly found organisms in plaque were tested for their capacity to induce lymphocyte transformation. There was a direct correlation between the severity of peridontal disease and the amount of transformation induced by the cell walls of oral bacteria and by solubilized dental plaque. Cord blood leukocytes and lymphocytes from clinically normal people did not respond, which indicates that these stimulants are antigens rather than mitogens. Of the eleven bacteria tested, four members of the family Actinomycetaceae (Actinomyces viscosus, A. israelii, A. naeslundii, and Arachnia propionica), the related Propionibacterium acnes, and an anaerobic gram-negative anaerobic rod (27N). The high prevalence of the former organisms in the mature dental plaque that forms around the gingival crevice area and the potent efficacy with which they stimulate lymphocytes indicates that Actinomyces and certain gram-negative anaerobes may be important etiological agents in chronic periodontal inflammation in man.

Topics: Actinomyces; Cell Wall; Concanavalin A; Dental Plaque; Gram-Negative Anaerobic Bacteria; Humans; In Vitro Techniques; Lymphocyte Activation; Periodontal Diseases