concanavalin-a and Osteoporosis

This study was designed to investigate the effects of dried plum on the changes in bone metabolism and the immune response associated with ovarian hormone deficiency. Adult female C57BL/6J mice were either sham-operated (Sham) and fed AIN-93 diet (control) or ovariectomized (OVX) and fed a control diet with 0%, 5%, 15% or 25% dried plum (w/w), corresponding to control, low- (LDP), medium- (MDP) and high (HDP)-dose dried plum. Four weeks of HDP supplementation prevented the decrease in spine bone mineral density and content induced by OVX. The OVX compromise in trabecular bone of the vertebra and proximal tibia was prevented by the higher doses of dried plum, and in the vertebra these effects resulted in greater (P<.05) bone strength and stiffness. In the bone marrow, OVX suppressed granulocyte and committed monocyte populations and increased the lymphoblast population, but the MDP and HDP restored these myeloid and lymphoid populations to the level of the Sham. Dried plum also suppressed lymphocyte tumor necrosis factor (TNF)-α production ex vivo by splenocytes, in response to concanavalin (Con) A stimulation. These data indicate that dried plum's positive effects on bone structural and biomechanical properties coincide with the restoration of certain bone marrow myeloid and lymphoid populations, and suppressed splenocyte activation occurring with ovarian hormone deficiency.

Topics: Animals; Biomarkers; Body Weight; Bone and Bones; Bone Density; Bone Marrow Cells; Concanavalin A; Cytokines; Dietary Supplements; Disease Models, Animal; Eating; Female; Femur; Gene Expression; Insulin-Like Growth Factor I; Mice; Mice, Inbred C57BL; Organ Size; Osteoporosis; Ovariectomy; Peptide Fragments; Procollagen; Prunus; Spleen; Tibia; Tumor Necrosis Factor-alpha; Uterus