concanavalin-a has been researched along with Neurodegenerative-Diseases* in 2 studies
2 other study(ies) available for concanavalin-a and Neurodegenerative-Diseases
Article | Year |
---|---|
To evaluate the preventive effect of SPRC on concanavalin A (Con A)-induced liver injury and uncover the underlying mechanisms.. Mice were randomly divided into five groups: control, Con A, SPRC (5 and 10 mg/kg injected intravenously once a day for 7 days), and propargylglycine (PAG; 50 mg/kg injected intraperitoneally 0.5 h before SPRC for 7 days). All mice except the controls were intravenously injected with Con A (20 mg/kg) on day 7. Alanine aminotransferase (ALT) and aspartate aminotransferase (AST) levels were evaluated using kits. Inflammatory cytokines (TNF-α and IFN-γ) in the blood and in the liver were detected by ELISA Kit and real-time PCR, respectively. The expression of mitogen-activated protein kinase (MAPK) pathway proteins (p-JNK and p-Akt) and apoptosis proteins (Bax and Bcl-2) was detected using western blotting.. SPRC reduced the levels of AST (. SPRC prevents Con A-induced liver injury in mice by promoting CSE expression and producing endogenous H Topics: Animals; Chemical and Drug Induced Liver Injury, Chronic; Concanavalin A; Cysteine; Cytokines; Hydrogen Sulfide; Mice; Neurodegenerative Diseases | 2022 |
Phasor-FLIM analysis of Thioflavin T self-quenching in Concanavalin amyloid fibrils.
The formation of amyloid structures has traditionally been related to human neurodegenerative pathologies and, in recent years, the interest in these highly stable nanostructures was extended to biomaterial sciences. A common method to monitor amyloid growth is the analysis of Thioflavin T fluorescence. The use of this highly selective dye, diffused worldwide, allows mechanistic studies of supramolecular assemblies also giving back important insight on the structure of these aggregates. Here we present experimental evidence of self-quenching effect of Thioflavin T in presence of amyloid fibrils. A significant reduction of fluorescence lifetime of this dye which is not related to the properties of analyzed amyloid structures is found. This result is achieved by coupling Fluorescence Lifetime Imaging Microscopy with phasor approach as suitable model-free methods and constitute a serious warning that have to be taken in account if is dye is used for quantitative studies. Topics: Amyloid; Benzothiazoles; Concanavalin A; Fluorescent Dyes; Humans; Inclusion Bodies; Microscopy, Fluorescence; Neurodegenerative Diseases; Optical Imaging; Staining and Labeling | 2020 |