concanavalin-a and Mycoses

Topics: Agammaglobulinemia; Animals; Antigens; Autoimmune Diseases; Binding, Competitive; Cell Transformation, Neoplastic; Chickens; Concanavalin A; Dysgammaglobulinemia; Epitopes; Genes; Humans; Immune Tolerance; Immunity, Cellular; Immunoglobulin A; Immunoglobulin Allotypes; Immunoglobulin E; Immunologic Deficiency Syndromes; Immunosuppression Therapy; Lymphokines; Mice; Mycoses; Rabbits; T-Lymphocytes

The authors investigated the efficacy of two fluorescein-conjugated lectins (FCLs), concanavalin A (F-ConA) and wheat germ agglutinin (F-WGA), to visualize microorganisms from clinical specimens with documented mycotic and acanthamoebic keratitis. Corneal scrapings from 18 patients with culture-proven keratomycosis and deparaffinized histopathologic specimens from five culture-proven cases of acanthamoebic keratitis were evaluated. The F-ConA provided consistently bright staining of the mycotic structures in each of the corneal scrapings. Both F-ConA and F-WGA stained acanthamoebic trophozoites and cysts in the histopathologic specimens. In both the corneal scrapings and the histopathologic specimens, the microorganisms were easily differentiated from background corneal cells and tissue. These staining patterns correlate well with the results from experimental studies, and with the known cell wall carbohydrate compositions for fungi and acanthamoebae. This study provides further evidence that FCLs (particularly F-ConA) may eventually become effective first-line stains for the visualization of microorganisms in specimens from ocular infections.

Topics: Acanthamoeba; Adolescent; Adult; Aged; Amebiasis; Animals; Child; Concanavalin A; Female; Fixatives; Fluoresceins; Fungi; Humans; Keratitis; Male; Microscopy, Fluorescence; Middle Aged; Mycoses; Wheat Germ Agglutinins

The ability of murine recombinant gamma interferon (IFN) or lymphokines to enhance the fungicidal activity of murine pulmonary macrophages (PuM) was studied in in vitro. PuM monolayers were incubated overnight with IFN, lymph node cells (LNC) plus concanavalin A, supernatants from Con A stimulated LNC or spleen cell cultures (Con A Sup), or tissue culture medium (TCM) +/- Con A (5 micrograms/ml) or +/- lipopolysaccharide (LPS, 10 ng to 10 micrograms/ml). After treatment, culture fluids were removed and PuM were challenged for 4 h with the yeast-form Blastomyces dermatitidis or 2 h with Candida albicans. Inoculum colony forming units (CFU) of B. dermatitidis were significantly reduced by PuM treated with 1000 U/ml of IFN (25 +/- 3%), Con A Sup (25 +/- 3%) or LNC plus Con A (37-44%), but not by TCM, ConA or LPS. Candida albicans was killed by PuM treated with Con A Sup (33 +/- 8%) or LNC plus Con A (30-43%), but not by TCM, Con A, or LPS, and the activity of Con A Sup was neutralized by anti-IFN antibody. Candida albicans was not significantly killed by PuM treated with IFN doses ranging from 1 to 10(5) U/ml; nor did addition of LPS to IFN, or prolonged (3 day) treatment with IFN, result in significant killing of C. albicans by PuM. However, IFN (100 U/ml) could activate resident peritoneal macrophages for significant candidacidal activity (63%). These data indicate that PuM can be activated for fungicidal activity, and that PuM differ from resident peritoneal macrophages with regard to induction of candidacidal activity by recombinant gamma-IFN.

Topics: Animals; Blastomycosis; Candidiasis; Concanavalin A; Interferon-gamma; Lipopolysaccharides; Lung; Lymph Nodes; Lymphokines; Macrophage Activation; Male; Mice; Mice, Inbred BALB C; Mycoses; Spleen

Topics: Agammaglobulinemia; Animals; Autoimmune Diseases; Chickens; Concanavalin A; Humans; Immunologic Deficiency Syndromes; Lymphocyte Cooperation; Macrophages; Mice; Mycoses; Neoplasms; Prostaglandins; T-Lymphocytes; T-Lymphocytes, Regulatory

Thymus-derived lymphocyte (T-cell) function, as determined in vivo by cutaneous reactivity to several antigens and in vitro by responsiveness to mitogens and antigens, was assessed in 14 patients infected with a variety of fungal organisms. While all patients manifested a normal frequency of peripheral blood T cells, only seven patients reacted to at least one of the antigens used for cutaneous testing and demonstrated normal in vitro T proliferative responses. Three patients exhibited cutaneous anergy but normal in vitro T-cell reactivity while four patients demonstrated persistent anergy and marked in vitro T-cell hyporeactivity which was independent of activity of infection, concurrent medication, or any associated disorders. The marked diminution of in vitro T-cell reactivity noted for these later four patients was not due to a deletion of antigen- or mitogen-reactive cells. Thus, patients' cells which had been initially cultured for 7 days without any mitogenic or antigenic stimulus and which were subsequently washed and recultured with phytohemagglutinin, concanavalin A, or histoplasmin demonstrated a marked increase in their responsiveness. Moreover, this reactivity noted for recultured cells could be suppressed by a nonphagocytic, nonadherent, nonimmunoglobulin-bearing, sheep red blood cell rosette-forming population of cells isolated from the fresh peripheral blood mononuclear cells of the same patient. While these "regulator" T cells were capable of suppressing T-proliferative responses to antigens and mitogens, they did not diminish pokeweed mitogen-induced immunoglobulin synthesis by normal bone marrow-derived lymphocytes. Patients in whom suppressor "T" cells were found were at risk for relapsing, disseminated fungal infection.

Topics: Antigen-Antibody Reactions; Antigens; B-Lymphocytes; Candida; Cells, Cultured; Concanavalin A; Histoplasmin; Humans; Immunoglobulins; Lectins; Monocytes; Mycoses; Recurrence