concanavalin-a and Laryngeal-Neoplasms

This study aims to evaluate the production of interferon-gamma and interleukin-10 by stimulated peripheral blood mononuclear cells isolated from patients with supraglottic laryngeal cancer before and after surgical treatment.. Fourteen patients with advanced supraglottic laryngeal cancer were studied. Cultures of peripheral blood mononuclear cells isolated during the preoperative and late postoperative periods were stimulated with concanavalin A and Bacille Calmette-Guerin, and the supernatant concentrations of interferon-gamma and interleukin-10 were measured.. For non-stimulated cultures, the interferon-gamma levels produced by the preoperative period and the late postoperative period cultures were lower than the levels produced by the control group cultures. The interferon-gamma levels after stimulation with concanavalin A were higher in the late postoperative period cultures than in the preoperative evaluation cultures. Stimulation with Bacille Calmette-Guerin led to the production of similar levels of interferon-gamma and interleukin-10 by all cultures; thus, stimulation increased the levels of interferon-gamma produced by both the preoperative and postoperative cultures relative to the levels produced by the corresponding unstimulated cultures.. Patients with advanced supraglottic laryngeal cancer exhibit an in vitro deficiency in interferongamma secretion by mononuclear cells. Stimulated cells seem to recover this function during the postoperative period.

Topics: Adolescent; Adult; Carcinoma; Case-Control Studies; Child; Concanavalin A; Cytokines; Female; Humans; Interferon-gamma; Interleukin-10; Laryngeal Neoplasms; Leukocytes, Mononuclear; Male; Mitogens; Mycobacterium bovis; Neoplasm Staging; Statistics, Nonparametric; Young Adult

The capacity of cell immunity to act against tumor cells has been presented as a decisive influence in the prognosis of patients with cancer. The aim of this study was to evaluate lymphoproliferation in nonadherent peripheral blood cell cultures of patients with advanced supraglottic laryngeal cancer.. Fourteen patients with advanced supraglottic laryngeal cancer were studied prospectively. Lymphoproliferation was quantified by adding 3H-thymidine and measured in counts/minute using liquid scintillation spectrometry. Based on the ratio between stimulated and baseline cultures, the proliferation index was calculated before and 236 +/- 18 days after the surgery.. Lymphoproliferation was lower in patients than in healthy controls (P = .01) in the preoperative as well as in the late postoperative period (P = .006 and P = .02, respectively). However, there was no change from preoperative to late postoperative.. Pre- and postoperative results show that patients with advanced supraglottic laryngeal cancer present lymphoproliferation diminished before the surgery, and in the late postoperative period, there was no recovery of immune capacity evaluated by lymphoproliferation measurement.

Topics: Adult; Aged; Carcinoma, Squamous Cell; Case-Control Studies; Concanavalin A; Female; Follow-Up Studies; Humans; Laryngeal Neoplasms; Leukocytes, Mononuclear; Lymphocyte Activation; Male; Middle Aged; Mitogens; Neoplasm Staging; Prospective Studies; Radiopharmaceuticals; Recovery of Function; Thymidine; Tritium

The in vitro responsiveness of peripheral blood mononuclear cells (PBMC) T lymphocytes was studied in 81 patients with limited or extended head and neck squamous cell carcinoma (HNSCC), as judged by T, N and T + N stages. Patients included in the study were males below 80 years of age, without auto-immune disease or cachexia, who were not taking any immuno-active medication at the time of diagnosis. The patients were divided into groups according to TNM stage T0-2 vs T3-4, N0-1 vs N2-3 or T + N0-3 vs T + N4-7. When cells from patients with early and late stage, according to T, N or T + N stage, were compared, we found a decreased level of mitogen stimulated T-cells and decreased spontaneous proliferation with increasing disease stage. The same was true if the in vitro mitogenesis of T-cells was analysed separately, depending on the laryngeal or oral cavity/pharyngeal origin of the patients' tumours. If the patients were divided into two groups based on N stage, decreased gamma-interferon, and to some extent interleukin (IL-2), but not IL-4 levels, were found to be related to the disease stage.

Topics: Aged; Analysis of Variance; Carcinoma, Squamous Cell; Cell Division; Concanavalin A; Head and Neck Neoplasms; Humans; Interferon-gamma; Interleukin-2; Interleukin-4; Laryngeal Neoplasms; Lymphocyte Activation; Male; Mitogens; Mouth Neoplasms; Multivariate Analysis; Neoplasm Staging; Pharyngeal Neoplasms; T-Lymphocytes

A comparison was made of HEp-2 cell surface changes induced by NDV or vaccinia virus infection. Three parameters were examined as a function of time after infection: the kinetics of hemadosorption and the appearance of concanavalin (con A) binding sites, and alterations in electrophoretic mobility of single cells. The kinetics of appearance of con A binding sites was strikingly similar for both virus infections, whereas hemadsorption preceded NDA synthesis and followed vaccinia synthesis. These data suggest that in the vaccinia-infected cell the hemadsorption and con A binding sites are different. NDV infection or exposure of sham-infected cells to bacterial neuraminidase significantly reduced their anodal mobilities. This also occurred after enzyme treatment of vaccinia-infected cells. Measurements of the sialic acid content of NDV or sham-infected cells before and after neuraminidase treatment indicated the exposure to the enzyme or NDV materially reduced the sialic acid content of cells. Vaccinia-infected cells contained considerably more sialic acid than did normal cells. For the vaccinia-infected cell a change in surface properties as detected by hemadsorption or increased con A binding was not reflected in a change in electrophoretic mobility.

Topics: Binding Sites; Carcinoma, Squamous Cell; Cell Line; Cell Membrane; Concanavalin A; Cytological Techniques; Electrophoresis; Hemadsorption; Kinetics; Laryngeal Neoplasms; Neuraminidase; Newcastle disease virus; Sialic Acids; Vaccinia virus; Virus Replication

Topics: Agglutination; Animals; Carcinoma; Carcinoma, Squamous Cell; Cell Line; Cell Transformation, Neoplastic; Concanavalin A; Cricetinae; Fibroblasts; HeLa Cells; Humans; Kidney; Laryngeal Neoplasms; Lectins; Lung; Mice; Mouth Neoplasms; Neoplasms, Experimental; Trypsin

Topics: Agglutination; Animals; Carcinoma; Carcinoma, Squamous Cell; Cell Line; Cell Membrane; Cell Transformation, Neoplastic; Chick Embryo; Concanavalin A; Cricetinae; Cycloheximide; Cytarabine; Floxuridine; Humans; Immune Sera; Kidney; Laryngeal Neoplasms; Lectins; Methods; Newcastle disease virus; Simplexvirus; Time Factors; Trypsin; Vitamin A

Topics: Acetylcholine; Adult; Brain; Brain Chemistry; Breast Neoplasms; Bronchial Neoplasms; Carcinoma; Cell Migration Inhibition; Concanavalin A; Epinephrine; Epitopes; Female; Histamine; Humans; Laryngeal Neoplasms; Lymphocytes; Macrophages; Male; Middle Aged; Myasthenia Gravis; Neoplasm Proteins; Neoplasms; Nerve Tissue Proteins; Norepinephrine; Sarcoidosis; Serotonin; Stomach Neoplasms; Succinylcholine; Tryptamines