concanavalin-a has been researched along with Heartwater-Disease* in 3 studies
3 other study(ies) available for concanavalin-a and Heartwater-Disease
| Article | Year |
|---|---|
Identification of Ehrlichia ruminantium (Gardel strain) IFN-gamma inducing proteins after vaccination with a killed vaccine.
IFN-gamma is considered as a key factor in protection against heartwater of ruminants, caused by the obligate intracellular bacterium Ehrlichia ruminantium. In this study, a better definition of the molecular masses of IFN-gamma inducing proteins of the Gardel strain of E. ruminantium was obtained by the use of continuous flow electrophoresis (CFE) and sensitized polyclonal lymphocytes. Out of 15 E. ruminantium CFE fractions tested within the 14-39 kDa region, eight were commonly reacted to by all goats. Interestingly, half of these fractions fall within the 23-29 kDa region, shown previously to contain polymorphic B-cell epitopes. Thus, the results suggest that this region also contains T-cell epitopes potentially involved in protection. Also, several proteins were found to be more immunogenic than the serologically immunodominant MAP1 protein. Finally, high activity within the 15-19 kDa region was observed, which confirms previous work done with CD4+ T-cell lines obtained from cattle immunized with a South African strain of E. ruminantium. The proteins falling within the molecular weight ranges defined in this study may have potential as vaccine antigens. Topics: Animals; Antigens, Protozoan; Concanavalin A; Ehrlichia ruminantium; Electrophoresis, Polyacrylamide Gel; Enzyme-Linked Immunosorbent Assay; Goat Diseases; Goats; Heartwater Disease; Interferon-gamma; Leukocytes, Mononuclear; Molecular Weight; Vaccination | 2004 |
Neutralization of bovine concanavalin-A T cell supernatant-mediated anti-Cowdria ruminantium activity with antibodies specific to interferon gamma but not to tumor necrosis factor.
In an earlier study we demonstrated that Concanavalin-A stimulated bovine T cell supernatants inhibited the growth of Cowdria ruminantium in bovine endothelial cells in vitro. An investigation was conducted to identify the cytokines which were responsible for this growth inhibition. Addition of antiserum against bovine interferon gamma (IFN gamma) reproducibly neutralized the inhibitory effect of the T cell supernatants, whereas addition of antisera against bovine tumor necrosis factor alpha (TNF alpha) had no effect. The inhibitory effect of IFN gamma on C. ruminantium growth was not mediated by the production of nitric oxide as there was no detectable difference in nitric oxide levels in cultures that were supplemented with T cell supernatants compared with those that were not. The IFN gamma mediated anti-C. ruminantium effect highlights the importance of cell mediated immune responses in control of these infections and in particular incriminates the protective role of T cells, or cells that secrete IFN gamma. Topics: Animals; Cattle; Cattle Diseases; Cells, Cultured; Concanavalin A; Ehrlichia ruminantium; Heartwater Disease; Immunity, Cellular; In Vitro Techniques; Interferon-gamma; Neutralization Tests; Nitric Oxide; T-Lymphocytes; Tumor Necrosis Factor-alpha | 1996 |
Conconavalin A-stimulated bovine T-cell supernatants inhibit growth of Cowdria ruminantium in bovine endothelial cells in vitro.
Conconavalin A-stimulated bovine T-cell supernatants inhibited the growth of Cowdria ruminantium in bovine endothelial cells in vitro but did not affect their entry. This finding represents one mechanism by which T cells may control C. ruminantium multiplication and hence affect the severity of disease. Topics: Animals; Cattle; Cell Line; Concanavalin A; Cytokines; Ehrlichia ruminantium; Endothelium, Vascular; Heartwater Disease; In Vitro Techniques; Interferon-gamma; T-Lymphocytes | 1994 |