concanavalin-a and Eczema

The effect of Fanetizole mesylate or CP-48,810, a new immunostimulating drug, on suppressor cell function and IgE synthesis in vitro was evaluated in atopic patients with allergic rhinitis and/or asthma and eczema. In the absence of the drug, histamine (10(-3)M) stimulated blood mononuclear cells from 23 atopic patients suppressed concanavalin A-induced lymphocyte proliferation by a mean (+/- S.E.M.) of 9.3% +/- 3.5 (compared to 25.1% +/- 2.7 for histamine stimulated mononuclear cells from non-atopic controls). The addition of the drug (2.5 X 10(-4)M) in vitro significantly increased histamine suppressor cell activity of atopic patients to 26.6% +/- 3.9 (compared to 24.7% +/- 2.8 for control cells in the presence of the drug). In order to determine a possible mechanism through which CP-48,810 might enhance histamine-induced suppressor activity, we examined the effects of the drug on the production of histamine-induced suppressor factor (HSF) by lymphocytes and the production of prostaglandin E2 by blood monocytes in the presence of HSF. Supernatants generated from histamine (10(-4)M) stimulated patient lymphocytes caused a 9.0% +/- 1.8 suppression of concanavalin A-induced lymphocyte proliferation (compared to 25.0% +/- 3.1 caused by supernatants from normal subjects). If the drug (2.5 X 10(-4)M) was added at the beginning of culture, HSF activity in supernatants derived from atopic lymphocytes increased significantly to 20.2% +/- 1.8 (compared to 23.3% +/- 3.9 for drug treated control supernatants). Prostaglandin E2 production by atopic monocytes exposed to HSF was less than that of normal monocytes in the absence of the drug.(ABSTRACT TRUNCATED AT 250 WORDS)

Topics: Adjuvants, Immunologic; Adolescent; Adult; Asthma; Cells, Cultured; Child; Concanavalin A; Dermatitis, Atopic; Dinoprostone; Eczema; Female; Histamine; Humans; Hypersensitivity, Immediate; Immunoglobulin E; Immunoglobulin G; Lymphocyte Activation; Male; Middle Aged; Monocytes; Prostaglandins E; Rhinitis; T-Lymphocytes, Regulatory; Thiazoles

Topics: Candida albicans; Candidiasis, Oral; Central Nervous System Diseases; Child, Preschool; Concanavalin A; Eczema; Epithelium; Humans; Infant; Lymphoma; Male; Phytohemagglutinins; Pokeweed Mitogens; Recurrence; Rosette Formation; Thymus Gland; Wiskott-Aldrich Syndrome

The effects of cyclophosphamide (200 microgram/ml), levamisole (1 microgram/ml) and Concanavalin A (10 microgram/ml) on the in vitro IgE biosynthesis of lymphocytes from 2 allergic children and six healthy adults were studied. The capability of the IgE biosynthesis was enhanced by cyclophosphamide in nine allergic children, suppressed by levamisole in three and not changed by Concanavalin A. The three patients who showed in vitro suppressed IgE synthesis by levamisole had been treated with levamisole for more than three months but only one revealed decreased serum IgE.

Topics: Adolescent; Asthma; Cells, Cultured; Child; Concanavalin A; Cyclophosphamide; Eczema; Female; Humans; Immunoglobulin E; Levamisole; Lymphocytes; Male; T-Lymphocytes

A technically simple procedure is described for simultaneous determination of numbers and functions of human blood lymphocyte subpopulations. The method is based on stimulation with mitogens (PHA, Con A and PWM) with simultaneous rosetting to detect E receptors and C3 receptors. Con A and PWM activate the affinity of C3 receptors in normal lymphocytes increasing the number of cells rosetting with zymosan-complement particles, and also the number of cells giving rosettes with both zymosan-complement particles and sheep red blood cells (B cells and 'D' cells respectively). PHA activates E receptors, increasing the number of T cells detected. The lymphocytes of 33 patients with allergic diseases of the skin and respiratory tract showed a decrease in C3 rosettes during stimulation with Con A and PWM, which suggests a defect of helper and suppressor functions of lymphocytes in these patients.

Topics: Asthma; B-Lymphocytes; Berylliosis; Concanavalin A; Eczema; Humans; Leukocyte Count; Lymphocyte Activation; Mitogens; Phytohemagglutinins; Pokeweed Mitogens; Rosette Formation; T-Lymphocytes

Topics: Adolescent; Adult; Cell Adhesion; Child; Concanavalin A; Cyclic AMP; Eczema; Female; Histamine; Humans; Immunoglobulin E; Immunosuppression Therapy; Lymphocyte Activation; Male; T-Lymphocytes