concanavalin-a and Cerebral-Hemorrhage

Premature infants with placental infection and adult stroke patients with fever have worse outcomes following intracerebral hemorrhage (ICH). We hypothesized that immune pre-activation would aggravate brain injury in mouse brain following ICH. The immune system of 2-day, 10-day and 7-week young adult CD1 mice was stimulated by intraperitoneal injection of concanavalin A (ConA), lipopolysaccharide (LPS) or polyinosinic-polycytidilic acid (PolyI:C) 12 h prior to intracerebral injection of blood. Two days later, brain damage and inflammation were worse in 2-day mice that had received LPS. The other agents had less consistent effects in 2-day mice. Brain damage in young adults was aggravated less after immune stimulation. These data suggest that immune pre-activation modifies hemorrhagic brain injury in immature mouse brain.

Topics: Aging; Animals; Animals, Newborn; Blood Transfusion; Brain; Cerebral Hemorrhage; Concanavalin A; Female; Injections, Intraperitoneal; Injections, Intraventricular; Interferon-gamma; Lipopolysaccharides; Lymphocyte Activation; Male; Mice; Mice, Inbred C57BL; Mice, Knockout; Poly I-C

Lymphocyte capping with concanavalin A was studied in 11 patients with hereditary cerebral haemorrhage with amyloidosis (Dutch type) and 10 controls. No difference in capping was found between patients and controls. Abnormal lymphocyte concanavalin A capping has been reported in patients with the Icelandic type of cerebral amyloidosis and in patients with Alzheimer's disease, a disease in which cerebral amyloid angiopathy can also be found. The results suggest a difference in pathogenesis between the Dutch type of cerebral amyloidosis and the other amyloid diseases.

Topics: Aged; Amyloidosis; Cerebral Hemorrhage; Colchicine; Concanavalin A; Dioxolanes; Female; Humans; Immunologic Capping; Lymphocytes; Male; Methods; Middle Aged